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Mixture of Antibody Arrays to Functionally Characterize Darkish Proteins in Human Olfactory Neuroepithelial Cells

The completion and annotation of the human proteome require the provision of knowledge associated to protein perform. Presently, greater than 1800 human genes represent the “darkish proteome,” which embody lacking proteins, uncharacterized human genes validated at protein degree, smORFs, proteins from lncRNAs, or any uncharacterized transcripts. Over the past years, totally different experimental workflows primarily based on multi-omics analyses, bioinformatics, and in vitro and in vivo research have been promoted by the Human Proteome Challenge Consortium to boost the annotation of darkish proteins.

On this chapter, we describe a technique that makes use of recombinant proteins and antibody arrays to ascertain an easy methodology in an effort to quickly characterize potential practical options of darkish proteins related to intracellular signaling dynamics and extracellular immune response in human cell cultures. Additional validating the strategy, this workflow was utilized to probe adjustments within the activation patterns of kinases and transcription components in addition to in cytokine manufacturing modulated by the darkish C1orf128 (PITHD1) protein in human olfactory neuroepithelial cells.

Identification of Antibody Biomarker Utilizing Excessive-Density Nucleic Acid Programmable Protein Array

A novel protein microarray expertise, known as high-density nucleic acid programmable protein array (HD-NAPPA), allows the serological screening of hundreds of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a standard glass microscope slide. By comparability, HD-NAPPA shows proteins in over 10,000 nanowells etched in a silicon slide.

Proteins on HD-NAPPA are expressed within the particular person remoted nanowells, through in vitro transcription and translation (IVTT), with none diffusion throughout incubation. Right here we describe the strategy for antibody biomarker identification utilizing HD-NAPPA, together with 4 major steps: (1) HD-NAPPA array protein expression, (2) main antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) picture scanning and information processing.

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SUFU Antibody

E309471 200ul
EUR 275
Description: Available in various conjugation types.

SUFU Antibody

DF7687 100ul
EUR 280
Description: Human

SUFU Antibody

DF7687-100ul 100ul
EUR 280

SUFU Antibody

DF7687-200ul 200ul
EUR 350

SUFU Antibody

E035993 100μg/100μl
EUR 255
Description: Available in various conjugation types.

SUFU Antibody

E19-7687-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

SUFU Antibody

E19-7687-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

SUFU antibody

E39-08373 100ug/100ul
EUR 225
Description: Available in various conjugation types.

SUFU antibody

E39-10794 100ug/100ul
EUR 225
Description: Available in various conjugation types.

anti- SUFU antibody

FNab08373 100µg
EUR 658.5
Description: Antibody raised against SUFU

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx238373-100ug 100 ug
EUR 610.8

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody

20-abx211712
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

20-abx322260
  • EUR 360.00
  • EUR 292.80
  • 100 ul
  • 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

20-abx127058
  • EUR 493.20
  • EUR 710.40
  • 100 ul
  • 200 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

20-abx005181
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

20-abx141906
  • EUR 444.00
  • EUR 727.20
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx145714-100ug 100 ug
EUR 469.2

SUFU (pS342) Antibody

20-abx000504
  • EUR 594.00
  • EUR 844.80
  • EUR 427.20
  • 100 ul
  • 200 ul
  • 50 ul

SUFU Conjugated Antibody

C39155 100ul
EUR 476.4

SUFU Conjugated Antibody

C35993 100ul
EUR 476.4

SUFU Polyclonal Antibody

E913429 100ul
EUR 225
Description: Available in various conjugation types.

SUFU Polyclonal Antibody

E96757 100ul
EUR 225
Description: Available in various conjugation types.

Polyclonal SUFU Antibody (C-term)

APR04889G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU (C-term). This antibody is tested and proven to work in the following applications:

Suppressor of Fused (SUFU) Antibody

E8RT1600 100ul
EUR 225
Description: Available in various conjugation types.

Monoclonal SUFU Antibody, Clone: 1783CT536.263.29

AMM02579G 0.1ml
EUR 580.8
Description: A Monoclonal antibody against Human SUFU. The antibodies are raised in Mouse and are from clone 1783CT536.263.29. This antibody is applicable in WB, E

Polyclonal SUFU antibody - middle region

APR00655G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications:

Polyclonal SUFU antibody - middle region

APR00749G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications:

Sufu (Phospho-Ser342) Antibody

11552-100ul 100ul
EUR 302.4

Sufu (Phospho-Ser342) Antibody

11552-50ul 50ul
EUR 224.4

Sufu (Phospho-Ser342) Antibody

E011552 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Polyclonal SUFU antibody - N-terminal region

APR00623G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - N-terminal region. This antibody is tested and proven to work in the following applications:

SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody

abx332917-100ul 100 ul
EUR 560.4

Monoclonal SUFU Antibody (monoclonal) (M01), Clone: 1B2

AMM04155G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human SUFU (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B2. This antibody is applicable in E

SUFU

E8ER1802-68 100ul
EUR 275
Description: Available in various conjugation types.

SUFU

pro-938 5µg
EUR 60
Description: Recombinant Human Suppressor of Fused Homolog

SUFU siRNA

20-abx935622
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

SUFU siRNA

20-abx935623
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

Sufu (Phospho-Ser342) Polyclonal Conjugated Antibody

C11552 100ul
EUR 476.4

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein

20-abx261500
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

SUFU Mouse mAb

E2618296 100ul
EUR 225
Description: Available in various conjugation types.

SUFU Rabbit pAb

A13429-100ul 100 ul
EUR 369.6

SUFU Rabbit pAb

A13429-200ul 200 ul
EUR 550.8

SUFU Rabbit pAb

A13429-20ul 20 ul
EUR 219.6

SUFU Rabbit pAb

A13429-50ul 50 ul
EUR 267.6

SUFU Rabbit pAb

A19438-100ul 100 ul Ask for price

SUFU Rabbit pAb

A19438-200ul 200 ul Ask for price

SUFU Rabbit pAb

A19438-20ul 20 ul Ask for price

SUFU Rabbit pAb

A19438-50ul 50 ul
EUR 369.6

SUFU Rabbit pAb

A6757-100ul 100 ul
EUR 369.6

SUFU Rabbit pAb

A6757-200ul 200 ul
EUR 550.8

SUFU Rabbit pAb

A6757-20ul 20 ul
EUR 219.6

SUFU Rabbit pAb

A6757-50ul 50 ul
EUR 267.6

SUFU Rabbit pAb

E2506757 100ul
EUR 225
Description: Available in various conjugation types.

SUFU Rabbit pAb

E2513429 100ul
EUR 225
Description: Available in various conjugation types.

Rabbit Anti-Human SUFU Polyclonal Antibody, Phospho-Ser342

CPB-869RH 100 ul
EUR 670.8

SUFU cloning plasmid

CSB-CL891560HU-10ug 10ug
EUR 620.4
Description: A cloning plasmid for the SUFU gene.

SUFU Blocking Peptide

DF7687-BP 1mg
EUR 234

Human SUFU Negative Regulator Of Hedgehog Signaling (SUFU) ELISA Kit

abx383558-96tests 96 tests
EUR 1093.2

SUFU protein (His tag)

80R-1977 100 ug
EUR 386.4
Description: Recombinant human SUFU protein (His tag)

SUFU ELISA KIT|Human

EF003347 96 Tests
EUR 826.8

Human SUFU shRNA Plasmid

20-abx959898
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse SUFU shRNA Plasmid

20-abx973629
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

SUFU Recombinant Protein (Rat)

RP231659 100 ug Ask for price

SUFU Recombinant Protein (Mouse)

RP176351 100 ug Ask for price

SUFU Recombinant Protein (Mouse)

RP176354 100 ug Ask for price

SUFU Recombinant Protein (Human)

RP030583 100 ug Ask for price

Sufu ORF Vector (Rat) (pORF)

ORF077221 1.0 ug DNA
EUR 607.2

SUFU (C-term) Rabbit pAb

E2619263 100ul
EUR 225
Description: Available in various conjugation types.

SUFU ORF Vector (Human) (pORF)

ORF010195 1.0 ug DNA
EUR 114

Sufu ORF Vector (Mouse) (pORF)

ORF058785 1.0 ug DNA
EUR 607.2

Sufu ORF Vector (Mouse) (pORF)

ORF058786 1.0 ug DNA
EUR 607.2

Human SUFU Protein Lysate 20ug

IHUSUFUPLLY20UG each
EUR 211
Description: Human SUFU Protein Lysate 20ug

Sufu sgRNA CRISPR Lentivector set (Rat)

K7545701 3 x 1.0 ug
EUR 406.8

Sufu 3'UTR GFP Stable Cell Line

TU271384 1.0 ml Ask for price

SUFU 3'UTR GFP Stable Cell Line

TU074900 1.0 ml
EUR 2799.6

Sufu 3'UTR GFP Stable Cell Line

TU169919 1.0 ml Ask for price

SUFU sgRNA CRISPR Lentivector set (Human)

K2311501 3 x 1.0 ug
EUR 406.8

Sufu sgRNA CRISPR Lentivector set (Mouse)

K4415301 3 x 1.0 ug
EUR 406.8

SUFU Protein Vector (Rat) (pPM-C-HA)

PV308884 500 ng
EUR 723.6

SUFU Protein Vector (Rat) (pPB-C-His)

PV308882 500 ng
EUR 723.6

SUFU Protein Vector (Rat) (pPB-N-His)

PV308883 500 ng
EUR 723.6

SUFU Protein Vector (Rat) (pPM-C-His)

PV308885 500 ng
EUR 723.6

SUFU Protein Vector (Human) (pPM-C-HA)

PV040779 500 ng
EUR 394.8

SUFU Protein Vector (Mouse) (pPM-C-HA)

PV235140 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPM-C-HA)

PV235144 500 ng
EUR 723.6

SUFU Protein Vector (Human) (pPB-C-His)

PV040777 500 ng
EUR 394.8

SUFU Protein Vector (Human) (pPB-N-His)

PV040778 500 ng
EUR 394.8

SUFU Protein Vector (Human) (pPM-C-His)

PV040780 500 ng
EUR 394.8

SUFU Protein Vector (Mouse) (pPB-C-His)

PV235138 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPB-N-His)

PV235139 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPM-C-His)

PV235141 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPB-C-His)

PV235142 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPB-N-His)

PV235143 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPM-C-His)

PV235145 500 ng
EUR 723.6

Sufu 3'UTR Luciferase Stable Cell Line

TU221384 1.0 ml Ask for price

Sufu 3'UTR Luciferase Stable Cell Line

TU119919 1.0 ml Ask for price

SUFU 3'UTR Luciferase Stable Cell Line

TU024900 1.0 ml
EUR 2799.6

Sufu sgRNA CRISPR Lentivector (Rat) (Target 1)

K7545702 1.0 ug DNA
EUR 184.8

Sufu sgRNA CRISPR Lentivector (Rat) (Target 2)

K7545703 1.0 ug DNA
EUR 184.8

Sufu sgRNA CRISPR Lentivector (Rat) (Target 3)

K7545704 1.0 ug DNA
EUR 184.8

SUFU sgRNA CRISPR Lentivector (Human) (Target 1)

K2311502 1.0 ug DNA
EUR 184.8

SUFU sgRNA CRISPR Lentivector (Human) (Target 2)

K2311503 1.0 ug DNA
EUR 184.8

SUFU sgRNA CRISPR Lentivector (Human) (Target 3)

K2311504 1.0 ug DNA
EUR 184.8

Sufu sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4415302 1.0 ug DNA
EUR 184.8

Sufu sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4415303 1.0 ug DNA
EUR 184.8

Sufu sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4415304 1.0 ug DNA
EUR 184.8

Recombinant Human SUFU Protein, His, E.coli-1mg

QP13639-1mg 1mg
EUR 3308.4

Recombinant Human SUFU Protein, His, E.coli-5ug

QP13639-5ug 5ug
EUR 186

Recombinant Human SUFU Protein, His, E.coli-20ug

QP13639-20ug 20ug
EUR 241.2

Anti-SUFU Antibody

A02279-1 100ug/vial
EUR 352.8

Anti-SUFU antibody

PAab08373 100 ug
EUR 463.2

Anti-SUFU Antibody

ER1802-68 100ul
EUR 189
Description: Su(fu) (for Suppressor-of-Fused) is a key negative regulator in the vertebrate Hedgehog signaling pathway. Su(fu) interacts with genes encoding proteins in this signal transduction pathway. In Drosophila, Intracellular transduction of the Hedgehog pathway involves the release of a large complex containing Su(fu). Su(fu) inhibits the activity of the transcription factor Gli1 and interacts with Gli2, Gli3 and the serine/threonine kinase Fused. Su(fu) is widely expressed in adult and embryonic tissues with higher expression in tissues patterned by hegdehog signaling. The Su(fu) gene locus maps to a region that is deleted in glioblastomas, prostate cancer, malignant melanoma and endometrial cancer.

Anti-SUFU antibody

STJ28840 100 µl
EUR 332.4
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Anti-SUFU antibody

STJ11100632 50 µl
EUR 344.4
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Anti-SUFU antibody

STJ115390 100 µl
EUR 332.4
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Mouse Suppressor of fused homolog, Sufu ELISA KIT

ELI-29930m 96 Tests
EUR 1038

Human Suppressor of fused homolog, SUFU ELISA KIT

ELI-18767h 96 Tests
EUR 988.8

SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV653185 1.0 ug DNA
EUR 818.4

SUFU Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV653189 1.0 ug DNA
EUR 818.4

SUFU Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV653190 1.0 ug DNA
EUR 818.4

SUFU Suppressor of Fused Homolog Human Recombinant Protein

PROTQ9UMX1 Regular: 20ug
EUR 380.4
Description: SUFU Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 504 amino acids (1-484 a.a.) and having a molecular mass of 56.1kDa.;SUFU is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Rabbit anti-SUFU Antibody

DL96331A-100ul 100 ul
EUR 334
Description: Suppressor of fused homolog; SUFUH

Rabbit anti-SUFU Antibody

DL96331A-50ul 50 ul
EUR 233.8
Description: Suppressor of fused homolog; SUFUH

Rabbit anti-SUFU Antibody

YLD7492-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to SUFU

Rabbit anti-SUFU Antibody

YLD7492-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to SUFU

Phospho-SUFU (Ser342) Antibody

CSB-PA229368- each
EUR 402
Description: A polyclonal antibody against Phospho-SUFU (Ser342). Recognizes Phospho-SUFU (Ser342) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

Phospho-SUFU (Ser342) Antibody

CSB-PA229368-100ul 100ul
EUR 434.4
Description: A polyclonal antibody against Phospho-SUFU (Ser342). Recognizes Phospho-SUFU (Ser342) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7545705 3 x 1.0 ug
EUR 451.2

SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K2311505 3 x 1.0 ug
EUR 451.2

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4415305 3 x 1.0 ug
EUR 451.2

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K7545706 1.0 ug DNA
EUR 200.4

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K7545707 1.0 ug DNA
EUR 200.4

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K7545708 1.0 ug DNA
EUR 200.4

Anti-Phospho-SUFU-(S342) antibody

STJ22424 100 µl
EUR 471.6
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K2311506 1.0 ug DNA
EUR 200.4

SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K2311507 1.0 ug DNA
EUR 200.4

SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K2311508 1.0 ug DNA
EUR 200.4

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4415306 1.0 ug DNA
EUR 200.4

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4415307 1.0 ug DNA
EUR 200.4

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4415308 1.0 ug DNA
EUR 200.4

SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV653186 1.0 ug DNA
EUR 818.4

SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV653187 1.0 ug DNA
EUR 888

SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV653188 1.0 ug DNA
EUR 888

Rabbit Anti SUFU Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot,Immunofluorescence)

IRBAMLSUFUAP632120UL each
EUR 346
Description: Rabbit Anti SUFU Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot,Immunofluorescence)

Rabbit Anti SUFU Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot,Immunofluorescence)

IRBAMLSUFUAP632200UL each
EUR 509
Description: Rabbit Anti SUFU Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot,Immunofluorescence)

Sufugolix

HY-100209 100mg
EUR 1614

Phospho-SUFU-S342 Rabbit pAb

AP0457-100ul 100 ul
EUR 460.8

Phospho-SUFU-S342 Rabbit pAb

AP0457-200ul 200 ul
EUR 664.8

Phospho-SUFU-S342 Rabbit pAb

AP0457-20ul 20 ul Ask for price

Phospho-SUFU-S342 Rabbit pAb

AP0457-50ul 50 ul
EUR 318

Antibody/Protein conjugation to FITC kit (sufficient for 2 reactions)

80230 1 kit
EUR 534

Ferret Primary Antibody (IgA) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80188-A 1 kit
EUR 781.2

Ferret Primary Antibody (IgG) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80188-G 1 kit
EUR 781.2

Ferret Primary Antibody (IgM) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80188-M 1 kit
EUR 781.2

mPEG antibody purification kit (mPEG based affinity matrix; sufficient to purify 10-20 mg of antibody from immunized animals) 10 ml column

PEG-AFP-10A 1 Kit
EUR 927.6

Hamster Serum Antibody detection ELISA kit, Qualitative (sufficient for 500-1000 tests)

80180 1 kit
EUR 781.2

Goat Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80165 1 Kit
EUR 781.2

Pig Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80186 1 kit
EUR 781.2

Human Primary Antibody (IgA) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80170-A 1 kit
EUR 781.2

Human Primary Antibody (IgE) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80170-E 1 kit
EUR 781.2

G. Pig Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80171 1 Kit
EUR 781.2

Sheep Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80166 1 Kit
EUR 781.2

Human Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80170 1 Kit
EUR 781.2

Donkey Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80172 1 Kit
EUR 781.2

Horse Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80173 1 Kit
EUR 781.2

Monkey Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80175 1 Kit
EUR 781.2

Bovine Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80185 1 kit
EUR 781.2

Turkey Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80187 1 kit
EUR 781.2

Ferret Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80188 1 kit
EUR 781.2

Human Primary Antibody (IgG1) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80170-G1 1 kit
EUR 781.2

Human Primary Antibody (IgG2) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80170-G2 1 kit
EUR 781.2

Chicken Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80176 1 kit
EUR 781.2

mPEG protein purification kit (Antibody based affinity matrix; sufficient to purify 2-4 mg protein) 2 ml column

PEG-AFP-02P 1 Kit
EUR 416.4

Chicken IgY Antibody (A+G+M) detection and titration in whole egg yolks ELISA kit, Qualitative (sufficient for 500-1000 tests)

80177 1 kit
EUR 781.2

Mouse Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-A 1 kit
EUR 781.2

Mouse Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-E 1 kit
EUR 781.2

Rat Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155-A 1 kit
EUR 781.2

Rat Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155-E 1 kit
EUR 781.2

Mouse Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150 1 Kit
EUR 781.2

Rat Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155 1 Kit
EUR 781.2

Rabbit Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80160 1 Kit
EUR 781.2

Mouse Primary Antibody (IgG1) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G1 1 kit
EUR 781.2

Mouse Primary Antibody (IgG2a) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G2a 1 kit
EUR 781.2

Conduction Band Power-Degree Engineering for Bettering Open-Circuit Voltage in Antimony Selenide Nanorod Array Photo voltaic Cells

Antimony selenide (Sb2 Se3 ) nanorod arrays alongside the [001] orientation are identified to switch photogenerated carriers quickly as a result of strongly anisotropic one-dimensional crystal construction. With superior light-trapping constructions, the Sb2 Se3 nanorod array-based photo voltaic cells have glorious broad spectral response properties, and better short-circuit present density than the traditional planar structured skinny movie photo voltaic cells. Nevertheless, the interface engineering for the Sb2 Se3 nanorod array-based photo voltaic cell is extra essential to extend the efficiency, as a result of it’s difficult to coat a compact buffer layer with good protection to type a uniform heterojunction interface attributable to its massive floor space and length-diameter ratio.

On this work, an intermeshing In2 S3 nanosheet-CdS composite because the buffer layer, compactly coating on the Sb2 Se3 nanorod floor is constructed. The appliance of In2 S3 -CdS composite buffers construct a gradient conduction band power configuration within the Sb2 Se3 /buffer heterojunction interface, which reduces the interface recombination and enhances the switch and assortment of photogenerated electrons. The energy-level regulation minimizes the open-circuit voltage deficit on the interfaces of buffer/Sb2 Se3 and buffer/ZnO layers within the Sb2 Se3 photo voltaic cells. Consequently, the Sb2 Se3 nanorod array photo voltaic cell primarily based on In2 S3 -CdS composite buffers achieves an effectivity of as excessive as 9.19% with a VOC of 461 mV.

Distinguishing between Isomeric Neoxanthin and Violaxanthin Esters in Yellow Flower Petals utilizing Liquid Chromatography-Photodiode Array Atmospheric Stress Chemical Ionization Mass Spectrometry and MS/MS

Rationale: Liquid chromatography-photodiode array atmospheric strain chemical ionization mass spectrometry (LC-PDA-APCI-MS) is used for the evaluation of assorted carotenoid pigments in crops. Amongst them, it’s tough to differentiate between the isomeric violaxanthin/neoxanthin esters.

Strategies: The yellow pigments of tomato petals have been extracted with acetone, and the extracts have been saved at -30°C to settle out the contaminating triacylglycerols bodily. The supernatants have been analyzed utilizing LC-PDA-APCI-MS with a high-resolution orbitrap MS for his or her precise lots. The anticipated carotenoid esters have been calculated with the mixture of carotenoids and fatty acids, they usually have been matched with the experimental precise lots. The fatty acid constructions within the carotenoid esters have been additionally recognized utilizing collision-induced dissociation (CID) MS/MS. The isomeric violaxanthin/neoxanthin esters have been distinguished utilizing CID MS/MS from their in-source dehydrated product ions as pseudoprecursor ions.

Outcomes: The in-source dehydrated ions [M-H2 O+H]+ of neoxanthin diesters predominated over their protonated molecules [M+H]+ within the LC-MS. Against this, the protonated molecules of violaxanthin diesters predominated. The 92 u loss product ions [M-H2 O-C7 H8 +H]+ have been noticed from the dehydrated violaxanthin diesters, however they weren’t generated from the dehydrated neoxanthin diesters within the CID MS/MS of their dehydrated pseudoprecursor ion [M-H2 O+H]+ .

Conclusions: The allene allyl carbocation in neoxanthin diesters was generated from dehydration after preferential protonation on the hydroxy group. The epoxide group of violaxanthin diesters opens simply after protonation; nonetheless, the dehydration didn’t proceed at this stage. The 92 u lack of C7 H8 was defined by the intramolecular [2+2] cycloaddition, which proceeded preferentially in dehydrated violaxanthin diesters as a result of the carbocations within the dehydrated species have been conjugated to the polyene and people double bonds have been depolarized within the CID MS/MS. Due to this fact, the isomeric neoxanthin/violaxanthin diesters have been distinguished utilizing LC-PDA-APCI-MS and MS/MS. This technique was a sensible and helpful technique of profiling the carotenoid esters of the yellow petals.

Beam pen lithography as a brand new software for spatially managed photochemistry, and its utilization within the synthesis of multivalent glycan arrays

Herein, we describe how cantilever-free scanning probes can be utilized to deposit precursor materials and subsequently irradiate the precursor to provoke polymerization, leading to a 3D lithographic technique whereby the place, peak and diameter of every function may be tuned independently. Particularly, acrylate and methacrylate monomers have been patterned onto thiol terminated glass and subsequently uncovered to UV mild produced brush polymers by a photoinduced radical acrylate polymerization response. Right here, we report the primary examples of glycan arrays, comprised of methacrylate brush polymers which are side-chain functionalized with α-glucose, by this new lithographic method.

Their binding with fluorophore labeled concanavalin A (ConA) was assayed by fluorescence microscopy. The fluorescence of those brush polymers was in comparison with glycan arrays composed of monolayers of α-mannosides and α-glucosides ready by combining polymer pen lithography (PPL) with the thiol-ene photochemical response or the copper-catalyzed azide-alkyne cycloaddition. At excessive ConA focus, the fluorescence sign of the brush polymer was practically 20 occasions better than that of the glycan monolayers, and the comb polymer arrays had a detection restrict practically two orders of magnitude higher than their monolayer counterparts.

Due to the potential of this technique to manage exactly the polymer size, the connection between restrict of detection and multivalency could possibly be explored, and it was discovered that the longer polymers (136 nm) are an order of magnitude extra delicate in the direction of ConA binding than the shorter polymers (Eight nm) and that binding affinity decreased systematically with size. These glycan arrays are a brand new software to check the function of multivalency on carbohydrate recognition, and the photopolymerization route in the direction of forming multivalent glycan scaffolds described herein, is a promising path to create multiplexed glycan arrays with nanoscale function dimensions.

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