Mixture of Antibody Arrays to Functionally Characterize Darkish Proteins in Human Olfactory Neuroepithelial Cells
The completion and annotation of the human proteome require the provision of knowledge associated to protein perform. Presently, greater than 1800 human genes represent the “darkish proteome,” which embody lacking proteins, uncharacterized human genes validated at protein degree, smORFs, proteins from lncRNAs, or any uncharacterized transcripts. Over the past years, totally different experimental workflows primarily based on multi-omics analyses, bioinformatics, and in vitro and in vivo research have been promoted by the Human Proteome Challenge Consortium to boost the annotation of darkish proteins.
On this chapter, we describe a technique that makes use of recombinant proteins and antibody arrays to ascertain an easy methodology in an effort to quickly characterize potential practical options of darkish proteins related to intracellular signaling dynamics and extracellular immune response in human cell cultures. Additional validating the strategy, this workflow was utilized to probe adjustments within the activation patterns of kinases and transcription components in addition to in cytokine manufacturing modulated by the darkish C1orf128 (PITHD1) protein in human olfactory neuroepithelial cells.
Identification of Antibody Biomarker Utilizing Excessive-Density Nucleic Acid Programmable Protein Array
A novel protein microarray expertise, known as high-density nucleic acid programmable protein array (HD-NAPPA), allows the serological screening of hundreds of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a standard glass microscope slide. By comparability, HD-NAPPA shows proteins in over 10,000 nanowells etched in a silicon slide.
Proteins on HD-NAPPA are expressed within the particular person remoted nanowells, through in vitro transcription and translation (IVTT), with none diffusion throughout incubation. Right here we describe the strategy for antibody biomarker identification utilizing HD-NAPPA, together with 4 major steps: (1) HD-NAPPA array protein expression, (2) main antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) picture scanning and information processing.

SUFU Conjugated Antibody |
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C35993 | SAB | 100ul | EUR 476.4 |
SUFU Conjugated Antibody |
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C39155 | SAB | 100ul | EUR 476.4 |
SUFU (pS342) Antibody |
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20-abx000504 | Abbexa |
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Anti-SUFU Antibody |
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A02279-1 | BosterBio | 100ug/vial | EUR 352.8 |
anti- SUFU antibody |
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FNab08373 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against SUFU |
Anti-SUFU antibody |
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PAab08373 | Lifescience Market | 100 ug | EUR 463.2 |
Anti-SUFU antibody |
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STJ11100632 | St John's Laboratory | 50 µl | EUR 344.4 |
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants. |
Anti-SUFU antibody |
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STJ28840 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants. |
Anti-SUFU antibody |
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STJ115390 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants. |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx145714-100ug | Abbexa | 100 ug | EUR 469.2 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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20-abx141906 | Abbexa |
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SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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20-abx127058 | Abbexa |
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SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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20-abx005181 | Abbexa |
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SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody |
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20-abx211712 | Abbexa |
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SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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20-abx322260 | Abbexa |
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SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx238373-100ug | Abbexa | 100 ug | EUR 610.8 |
SUFU siRNA |
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20-abx935622 | Abbexa |
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SUFU siRNA |
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20-abx935623 | Abbexa |
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Phospho-SUFU (Ser342) Antibody |
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CSB-PA229368-100ul | Cusabio | 100ul | EUR 434.4 |
Description: A polyclonal antibody against Phospho-SUFU (Ser342). Recognizes Phospho-SUFU (Ser342) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
Sufu (Phospho-Ser342) Antibody |
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11552-100ul | SAB | 100ul | EUR 302.4 |
Sufu (Phospho-Ser342) Antibody |
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11552-50ul | SAB | 50ul | EUR 224.4 |
Phospho-SUFU (Ser342) Antibody |
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CSB-PA229368- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against Phospho-SUFU (Ser342). Recognizes Phospho-SUFU (Ser342) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein |
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20-abx261500 | Abbexa |
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SUFU cloning plasmid |
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CSB-CL891560HU-10ug | Cusabio | 10ug | EUR 620.4 |
Description: A cloning plasmid for the SUFU gene. |
SUFU Blocking Peptide |
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DF7687-BP | Affbiotech | 1mg | EUR 234 |
SUFU Rabbit pAb |
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A13429-100ul | Abclonal | 100 ul | EUR 369.6 |
SUFU Rabbit pAb |
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A13429-200ul | Abclonal | 200 ul | EUR 550.8 |
SUFU Rabbit pAb |
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A13429-20ul | Abclonal | 20 ul | EUR 219.6 |
SUFU Rabbit pAb |
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A13429-50ul | Abclonal | 50 ul | EUR 267.6 |
SUFU Rabbit pAb |
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A19438-100ul | Abclonal | 100 ul | Ask for price |
SUFU Rabbit pAb |
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A19438-200ul | Abclonal | 200 ul | Ask for price |
SUFU Rabbit pAb |
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A19438-20ul | Abclonal | 20 ul | Ask for price |
SUFU Rabbit pAb |
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A19438-50ul | Abclonal | 50 ul | EUR 369.6 |
SUFU Rabbit pAb |
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A6757-100ul | Abclonal | 100 ul | EUR 369.6 |
SUFU Rabbit pAb |
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A6757-200ul | Abclonal | 200 ul | EUR 550.8 |
SUFU Rabbit pAb |
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A6757-20ul | Abclonal | 20 ul | EUR 219.6 |
SUFU Rabbit pAb |
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A6757-50ul | Abclonal | 50 ul | EUR 267.6 |
SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody |
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abx332917-100ul | Abbexa | 100 ul | EUR 560.4 |
Monoclonal SUFU Antibody, Clone: 1783CT536.263.29 |
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AMM02579G | Leading Biology | 0.1ml | EUR 580.8 |
Description: A Monoclonal antibody against Human SUFU. The antibodies are raised in Mouse and are from clone 1783CT536.263.29. This antibody is applicable in WB, E |
Polyclonal SUFU Antibody (C-term) |
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APR04889G | Leading Biology | 0.1ml | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU (C-term). This antibody is tested and proven to work in the following applications: |
Polyclonal SUFU antibody - middle region |
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APR00655G | Leading Biology | 0.05mg | EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications: |
Polyclonal SUFU antibody - middle region |
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APR00749G | Leading Biology | 0.05mg | EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications: |
Anti-Phospho-SUFU-(S342) antibody |
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STJ22424 | St John's Laboratory | 100 µl | EUR 471.6 |
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants. |
Human SUFU Negative Regulator Of Hedgehog Signaling (SUFU) ELISA Kit |
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abx383558-96tests | Abbexa | 96 tests | EUR 1093.2 |
Human SUFU shRNA Plasmid |
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20-abx959898 | Abbexa |
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Mouse SUFU shRNA Plasmid |
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20-abx973629 | Abbexa |
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SUFU protein (His tag) |
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80R-1977 | Fitzgerald | 100 ug | EUR 386.4 |
Description: Recombinant human SUFU protein (His tag) |
SUFU ELISA KIT|Human |
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EF003347 | Lifescience Market | 96 Tests | EUR 826.8 |
SUFU Recombinant Protein (Human) |
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RP030583 | ABM | 100 ug | Ask for price |
SUFU Recombinant Protein (Rat) |
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RP231659 | ABM | 100 ug | Ask for price |
SUFU Recombinant Protein (Mouse) |
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RP176351 | ABM | 100 ug | Ask for price |
SUFU Recombinant Protein (Mouse) |
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RP176354 | ABM | 100 ug | Ask for price |
Sufu (Phospho-Ser342) Polyclonal Conjugated Antibody |
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C11552 | SAB | 100ul | EUR 476.4 |
Polyclonal SUFU antibody - N-terminal region |
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APR00623G | Leading Biology | 0.05mg | EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - N-terminal region. This antibody is tested and proven to work in the following applications: |
Phospho-SUFU-S342 Rabbit pAb |
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AP0457-100ul | Abclonal | 100 ul | EUR 460.8 |
Phospho-SUFU-S342 Rabbit pAb |
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AP0457-200ul | Abclonal | 200 ul | EUR 664.8 |
Phospho-SUFU-S342 Rabbit pAb |
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AP0457-20ul | Abclonal | 20 ul | Ask for price |
Phospho-SUFU-S342 Rabbit pAb |
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AP0457-50ul | Abclonal | 50 ul | EUR 318 |
Sufu ORF Vector (Rat) (pORF) |
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ORF077221 | ABM | 1.0 ug DNA | EUR 607.2 |
Sufu ORF Vector (Mouse) (pORF) |
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ORF058785 | ABM | 1.0 ug DNA | EUR 607.2 |
Sufu ORF Vector (Mouse) (pORF) |
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ORF058786 | ABM | 1.0 ug DNA | EUR 607.2 |
SUFU ORF Vector (Human) (pORF) |
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ORF010195 | ABM | 1.0 ug DNA | EUR 114 |
Monoclonal SUFU Antibody (monoclonal) (M01), Clone: 1B2 |
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AMM04155G | Leading Biology | 0.1mg | EUR 580.8 |
Description: A Monoclonal antibody against Human SUFU (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B2. This antibody is applicable in E |
Rabbit Anti-Human SUFU Polyclonal Antibody, Phospho-Ser342 |
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CPB-869RH | Creative Diagnostics | 100 ul | EUR 670.8 |
SUFU sgRNA CRISPR Lentivector set (Human) |
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K2311501 | ABM | 3 x 1.0 ug | EUR 406.8 |
Sufu sgRNA CRISPR Lentivector set (Mouse) |
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K4415301 | ABM | 3 x 1.0 ug | EUR 406.8 |
Sufu sgRNA CRISPR Lentivector set (Rat) |
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K7545701 | ABM | 3 x 1.0 ug | EUR 406.8 |
SUFU sgRNA CRISPR Lentivector (Human) (Target 1) |
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K2311502 | ABM | 1.0 ug DNA | EUR 184.8 |
SUFU sgRNA CRISPR Lentivector (Human) (Target 2) |
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K2311503 | ABM | 1.0 ug DNA | EUR 184.8 |
SUFU sgRNA CRISPR Lentivector (Human) (Target 3) |
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K2311504 | ABM | 1.0 ug DNA | EUR 184.8 |
Sufu sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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K4415302 | ABM | 1.0 ug DNA | EUR 184.8 |
Sufu sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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K4415303 | ABM | 1.0 ug DNA | EUR 184.8 |
Sufu sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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K4415304 | ABM | 1.0 ug DNA | EUR 184.8 |
Sufu sgRNA CRISPR Lentivector (Rat) (Target 1) |
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K7545702 | ABM | 1.0 ug DNA | EUR 184.8 |
Sufu sgRNA CRISPR Lentivector (Rat) (Target 2) |
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K7545703 | ABM | 1.0 ug DNA | EUR 184.8 |
Sufu sgRNA CRISPR Lentivector (Rat) (Target 3) |
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K7545704 | ABM | 1.0 ug DNA | EUR 184.8 |
SUFU Protein Vector (Human) (pPB-C-His) |
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PV040777 | ABM | 500 ng | EUR 394.8 |
SUFU Protein Vector (Human) (pPB-N-His) |
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PV040778 | ABM | 500 ng | EUR 394.8 |
SUFU Protein Vector (Human) (pPM-C-HA) |
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PV040779 | ABM | 500 ng | EUR 394.8 |
SUFU Protein Vector (Human) (pPM-C-His) |
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PV040780 | ABM | 500 ng | EUR 394.8 |
Recombinant Human SUFU Protein, His, E.coli-1mg |
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QP13639-1mg | EnQuireBio | 1mg | EUR 3308.4 |
Recombinant Human SUFU Protein, His, E.coli-20ug |
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QP13639-20ug | EnQuireBio | 20ug | EUR 241.2 |
Recombinant Human SUFU Protein, His, E.coli-5ug |
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QP13639-5ug | EnQuireBio | 5ug | EUR 186 |
SUFU Protein Vector (Mouse) (pPB-C-His) |
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PV235138 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPB-N-His) |
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PV235139 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPM-C-HA) |
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PV235140 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPM-C-His) |
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PV235141 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPB-C-His) |
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PV235142 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPB-N-His) |
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PV235143 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPM-C-HA) |
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PV235144 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPM-C-His) |
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PV235145 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Rat) (pPB-C-His) |
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PV308882 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Rat) (pPB-N-His) |
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PV308883 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Rat) (pPM-C-HA) |
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PV308884 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Rat) (pPM-C-His) |
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PV308885 | ABM | 500 ng | EUR 723.6 |
Sufu 3'UTR Luciferase Stable Cell Line |
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TU221384 | ABM | 1.0 ml | Ask for price |
Sufu 3'UTR GFP Stable Cell Line |
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TU169919 | ABM | 1.0 ml | Ask for price |
Sufu 3'UTR GFP Stable Cell Line |
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TU271384 | ABM | 1.0 ml | Ask for price |
Sufu 3'UTR Luciferase Stable Cell Line |
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TU119919 | ABM | 1.0 ml | Ask for price |
SUFU 3'UTR GFP Stable Cell Line |
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TU074900 | ABM | 1.0 ml | EUR 2799.6 |
SUFU 3'UTR Luciferase Stable Cell Line |
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TU024900 | ABM | 1.0 ml | EUR 2799.6 |
SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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LV653185 | ABM | 1.0 ug DNA | EUR 818.4 |
SUFU Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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LV653189 | ABM | 1.0 ug DNA | EUR 818.4 |
SUFU Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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LV653190 | ABM | 1.0 ug DNA | EUR 818.4 |
Mouse Suppressor of fused homolog, Sufu ELISA KIT |
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ELI-29930m | Lifescience Market | 96 Tests | EUR 1038 |
Human Suppressor of fused homolog, SUFU ELISA KIT |
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ELI-18767h | Lifescience Market | 96 Tests | EUR 988.8 |
SUFU Suppressor of Fused Homolog Human Recombinant Protein |
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PROTQ9UMX1 | BosterBio | Regular: 20ug | EUR 380.4 |
Description: SUFU Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 504 amino acids (1-484 a.a.) and having a molecular mass of 56.1kDa.;SUFU is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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K2311505 | ABM | 3 x 1.0 ug | EUR 451.2 |
Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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K4415305 | ABM | 3 x 1.0 ug | EUR 451.2 |
Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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K7545705 | ABM | 3 x 1.0 ug | EUR 451.2 |
SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV653186 | ABM | 1.0 ug DNA | EUR 818.4 |
SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV653187 | ABM | 1.0 ug DNA | EUR 888 |
SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV653188 | ABM | 1.0 ug DNA | EUR 888 |
SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) |
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K2311506 | ABM | 1.0 ug DNA | EUR 200.4 |
SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2) |
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K2311507 | ABM | 1.0 ug DNA | EUR 200.4 |
SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) |
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K2311508 | ABM | 1.0 ug DNA | EUR 200.4 |
Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1) |
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K4415306 | ABM | 1.0 ug DNA | EUR 200.4 |
Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2) |
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K4415307 | ABM | 1.0 ug DNA | EUR 200.4 |
Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3) |
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K4415308 | ABM | 1.0 ug DNA | EUR 200.4 |
Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1) |
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K7545706 | ABM | 1.0 ug DNA | EUR 200.4 |
Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2) |
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K7545707 | ABM | 1.0 ug DNA | EUR 200.4 |
Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3) |
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K7545708 | ABM | 1.0 ug DNA | EUR 200.4 |
Positive control tissue section for each individua |
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Control-Slides-for-each-antibody | Innovex | Set of 25 | EUR 355 |
Description: Positive control tissue section for each individual antibody; Based on availability; INQUIRE |
ASAP1 antibody Antibody |
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DF8746 | Affbiotech | 200ul | EUR 420 |
CD11b Antibody Antibody |
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ABD2911 | Lifescience Market | 100 ug | EUR 525.6 |
anti- Antibody^Polyclonal antibody control antibody |
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LSMab09882 | Lifescience Market | 100 ug | EUR 525.6 |
ARHGDIA Antibody / RHOGDI Antibody |
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F54788-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 165 |
ARHGDIA Antibody / RHOGDI Antibody |
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F54788-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 379 |
Antibody |
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A1360-500 | Biovision | each | Ask for price |
Ly1 Antibody Reactive (LYAR) Antibody |
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20-abx123734 | Abbexa |
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Anti-Glycolipid Antibody (AGA) Antibody |
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20-abx004855 | Abbexa |
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Ly1 Antibody Reactive (LYAR) Antibody |
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20-abx014333 | Abbexa |
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Ly1 Antibody Reactive (LYAR) Antibody |
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20-abx008109 | Abbexa |
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Ly1 Antibody Reactive (LYAR) Antibody |
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abx033330-400ul | Abbexa | 400 ul | EUR 627.6 |
Ly1 Antibody Reactive (LYAR) Antibody |
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abx033330-80l | Abbexa | 80 µl | EUR 343.2 |
Anti-Glycolipid Antibody (AGA) Antibody |
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abx036399-100ug | Abbexa | 100 ug | EUR 469.2 |
Anti-Glycolipid Antibody (AGA) Antibody |
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abx230204-100ug | Abbexa | 100 ug | EUR 577.2 |
Anti-Glycoprotein Antibody (GP) Antibody |
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20-abx319900 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody |
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20-abx319901 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody |
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20-abx319905 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody |
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20-abx319913 | Abbexa |
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Ly1 Antibody Reactive (LYAR) Antibody |
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20-abx311665 | Abbexa |
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Ly1 Antibody Reactive (LYAR) Antibody |
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20-abx324434 | Abbexa |
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Ly1 Antibody Reactive (LYAR) Antibody |
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abx234901-100ug | Abbexa | 100 ug | EUR 661.2 |
Anti-Anti-SEPT6 antibody antibody |
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STJ11100949 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. |
Anti-Anti-SEPT9 Antibody antibody |
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STJ111369 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described. |
Anti-Anti-SEPT11 Antibody antibody |
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STJ111530 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT4 Antibody antibody |
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STJ112276 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. |
Anti-Anti-MARCH9 Antibody antibody |
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STJ112609 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT2 Antibody antibody |
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STJ25475 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT5 Antibody antibody |
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STJ25477 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
Anti-Anti-SEPT8 Antibody antibody |
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STJ25479 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-SEPT2 Antibody antibody |
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STJ28365 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT7 Antibody antibody |
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STJ28963 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
Anti-Anti-SEPT1 antibody antibody |
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STJ119580 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012] |
Anti-Anti-SEPT12 Antibody antibody |
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STJ117759 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-MARCH6 Antibody antibody |
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STJ118549 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-MARCH6 Antibody antibody |
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STJ118550 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-MARCH7 Antibody antibody |
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STJ118752 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT3 Antibody antibody |
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STJ118990 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT11 Antibody antibody |
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STJ113941 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT11 Antibody antibody |
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STJ114081 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT5 Antibody antibody |
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STJ114819 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
Anti-Anti-MARCH8 Antibody antibody |
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STJ114828 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT7 Antibody antibody |
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STJ116214 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
Anti-Anti-SEPT8 Antibody antibody |
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STJ117206 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
CLCN5 Antibody / CIC-5 antibody |
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RQ6462 | NSJ Bioreagents | 100ug | EUR 419 |
Description: The CLCN5 gene encodes the chloride channel Cl-/H+ exchanger ClC-5. This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. |
Cytokeratin 7 antibody-Cytoskeleton Marker Antibody |
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48169-100ul | SAB | 100ul | EUR 399.6 |
Cytokeratin 7 antibody-Cytoskeleton Marker Antibody |
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48169-50ul | SAB | 50ul | EUR 286.8 |
Antibody Pair to ApoA-V antibody |
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10R-1876 | Fitzgerald | 100 ul | EUR 781.2 |
Description: Mouse monoclonal Antibody Pair to ApoA-V antibody |
Anti CD22 Antibody: CD22 Monoclonal Antibody |
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065-A-01mg | Virogen | 0,1 mg | EUR 321 |
Description: anti-CD22 monoclonal antibody |
Anti CD22 Antibody: CD22 Monoclonal Antibody |
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065-A-1000ug | Virogen | 1000 ug | EUR 1539 |
Description: anti-CD22 monoclonal antibody |
Ly1 Antibody Reactive Homolog (LYAR) Antibody |
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20-abx103034 | Abbexa |
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Ly1 Antibody Reactive Homolog (LYAR) Antibody |
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20-abx103035 | Abbexa |
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Ly1 Antibody Reactive Homolog (LYAR) Antibody |
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20-abx103036 | Abbexa |
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Hepatitis C Virus Antibody (HCV) Antibody |
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abx023924-1mg | Abbexa | 1 mg | EUR 1446 |
Anti-Glycoprotein 210 Antibody (gp210) Antibody |
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abx233571-100ug | Abbexa | 100 ug | EUR 577.2 |
Anti Deoxyribonucleic Acid Antibody (DNA) Antibody |
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abx411057-50ug | Abbexa | 50 ug | EUR 710.4 |
Anti-Glycoprotein Antibody (GP) Antibody (HRP) |
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20-abx319902 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (FITC) |
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20-abx319903 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (Biotin) |
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20-abx319904 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (HRP) |
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20-abx319906 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (FITC) |
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20-abx319907 | Abbexa |
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Conduction Band Power-Degree Engineering for Bettering Open-Circuit Voltage in Antimony Selenide Nanorod Array Photo voltaic Cells
Antimony selenide (Sb2 Se3 ) nanorod arrays alongside the [001] orientation are identified to switch photogenerated carriers quickly as a result of strongly anisotropic one-dimensional crystal construction. With superior light-trapping constructions, the Sb2 Se3 nanorod array-based photo voltaic cells have glorious broad spectral response properties, and better short-circuit present density than the traditional planar structured skinny movie photo voltaic cells. Nevertheless, the interface engineering for the Sb2 Se3 nanorod array-based photo voltaic cell is extra essential to extend the efficiency, as a result of it’s difficult to coat a compact buffer layer with good protection to type a uniform heterojunction interface attributable to its massive floor space and length-diameter ratio.
On this work, an intermeshing In2 S3 nanosheet-CdS composite because the buffer layer, compactly coating on the Sb2 Se3 nanorod floor is constructed. The appliance of In2 S3 -CdS composite buffers construct a gradient conduction band power configuration within the Sb2 Se3 /buffer heterojunction interface, which reduces the interface recombination and enhances the switch and assortment of photogenerated electrons. The energy-level regulation minimizes the open-circuit voltage deficit on the interfaces of buffer/Sb2 Se3 and buffer/ZnO layers within the Sb2 Se3 photo voltaic cells. Consequently, the Sb2 Se3 nanorod array photo voltaic cell primarily based on In2 S3 -CdS composite buffers achieves an effectivity of as excessive as 9.19% with a VOC of 461 mV.
Distinguishing between Isomeric Neoxanthin and Violaxanthin Esters in Yellow Flower Petals utilizing Liquid Chromatography-Photodiode Array Atmospheric Stress Chemical Ionization Mass Spectrometry and MS/MS
Rationale: Liquid chromatography-photodiode array atmospheric strain chemical ionization mass spectrometry (LC-PDA-APCI-MS) is used for the evaluation of assorted carotenoid pigments in crops. Amongst them, it’s tough to differentiate between the isomeric violaxanthin/neoxanthin esters.
Strategies: The yellow pigments of tomato petals have been extracted with acetone, and the extracts have been saved at -30°C to settle out the contaminating triacylglycerols bodily. The supernatants have been analyzed utilizing LC-PDA-APCI-MS with a high-resolution orbitrap MS for his or her precise lots. The anticipated carotenoid esters have been calculated with the mixture of carotenoids and fatty acids, they usually have been matched with the experimental precise lots. The fatty acid constructions within the carotenoid esters have been additionally recognized utilizing collision-induced dissociation (CID) MS/MS. The isomeric violaxanthin/neoxanthin esters have been distinguished utilizing CID MS/MS from their in-source dehydrated product ions as pseudoprecursor ions.
Outcomes: The in-source dehydrated ions [M-H2 O+H]+ of neoxanthin diesters predominated over their protonated molecules [M+H]+ within the LC-MS. Against this, the protonated molecules of violaxanthin diesters predominated. The 92 u loss product ions [M-H2 O-C7 H8 +H]+ have been noticed from the dehydrated violaxanthin diesters, however they weren’t generated from the dehydrated neoxanthin diesters within the CID MS/MS of their dehydrated pseudoprecursor ion [M-H2 O+H]+ .
Conclusions: The allene allyl carbocation in neoxanthin diesters was generated from dehydration after preferential protonation on the hydroxy group. The epoxide group of violaxanthin diesters opens simply after protonation; nonetheless, the dehydration didn’t proceed at this stage. The 92 u lack of C7 H8 was defined by the intramolecular [2+2] cycloaddition, which proceeded preferentially in dehydrated violaxanthin diesters as a result of the carbocations within the dehydrated species have been conjugated to the polyene and people double bonds have been depolarized within the CID MS/MS. Due to this fact, the isomeric neoxanthin/violaxanthin diesters have been distinguished utilizing LC-PDA-APCI-MS and MS/MS. This technique was a sensible and helpful technique of profiling the carotenoid esters of the yellow petals.
Beam pen lithography as a brand new software for spatially managed photochemistry, and its utilization within the synthesis of multivalent glycan arrays
Herein, we describe how cantilever-free scanning probes can be utilized to deposit precursor materials and subsequently irradiate the precursor to provoke polymerization, leading to a 3D lithographic technique whereby the place, peak and diameter of every function may be tuned independently. Particularly, acrylate and methacrylate monomers have been patterned onto thiol terminated glass and subsequently uncovered to UV mild produced brush polymers by a photoinduced radical acrylate polymerization response. Right here, we report the primary examples of glycan arrays, comprised of methacrylate brush polymers which are side-chain functionalized with α-glucose, by this new lithographic method.
Their binding with fluorophore labeled concanavalin A (ConA) was assayed by fluorescence microscopy. The fluorescence of those brush polymers was in comparison with glycan arrays composed of monolayers of α-mannosides and α-glucosides ready by combining polymer pen lithography (PPL) with the thiol-ene photochemical response or the copper-catalyzed azide-alkyne cycloaddition. At excessive ConA focus, the fluorescence sign of the brush polymer was practically 20 occasions better than that of the glycan monolayers, and the comb polymer arrays had a detection restrict practically two orders of magnitude higher than their monolayer counterparts.
Due to the potential of this technique to manage exactly the polymer size, the connection between restrict of detection and multivalency could possibly be explored, and it was discovered that the longer polymers (136 nm) are an order of magnitude extra delicate in the direction of ConA binding than the shorter polymers (Eight nm) and that binding affinity decreased systematically with size. These glycan arrays are a brand new software to check the function of multivalency on carbohydrate recognition, and the photopolymerization route in the direction of forming multivalent glycan scaffolds described herein, is a promising path to create multiplexed glycan arrays with nanoscale function dimensions.