Mixture of Antibody Arrays to Functionally Characterize Darkish Proteins in Human Olfactory Neuroepithelial Cells
The completion and annotation of the human proteome require the provision of knowledge associated to protein perform. Presently, greater than 1800 human genes represent the “darkish proteome,” which embody lacking proteins, uncharacterized human genes validated at protein degree, smORFs, proteins from lncRNAs, or any uncharacterized transcripts. Over the past years, totally different experimental workflows primarily based on multi-omics analyses, bioinformatics, and in vitro and in vivo research have been promoted by the Human Proteome Challenge Consortium to boost the annotation of darkish proteins.
On this chapter, we describe a technique that makes use of recombinant proteins and antibody arrays to ascertain an easy methodology in an effort to quickly characterize potential practical options of darkish proteins related to intracellular signaling dynamics and extracellular immune response in human cell cultures. Additional validating the strategy, this workflow was utilized to probe adjustments within the activation patterns of kinases and transcription components in addition to in cytokine manufacturing modulated by the darkish C1orf128 (PITHD1) protein in human olfactory neuroepithelial cells.
Identification of Antibody Biomarker Utilizing Excessive-Density Nucleic Acid Programmable Protein Array
A novel protein microarray expertise, known as high-density nucleic acid programmable protein array (HD-NAPPA), allows the serological screening of hundreds of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a standard glass microscope slide. By comparability, HD-NAPPA shows proteins in over 10,000 nanowells etched in a silicon slide.
Proteins on HD-NAPPA are expressed within the particular person remoted nanowells, through in vitro transcription and translation (IVTT), with none diffusion throughout incubation. Right here we describe the strategy for antibody biomarker identification utilizing HD-NAPPA, together with 4 major steps: (1) HD-NAPPA array protein expression, (2) main antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) picture scanning and information processing.
SUFU Antibody |
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DF7687-200ul | Affinity Biosciences | 200ul | EUR 350 |
SUFU antibody |
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E39-08373 | EnoGene | 100ug/100ul | EUR 225 |
Description: Available in various conjugation types. |
SUFU antibody |
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E39-10794 | EnoGene | 100ug/100ul | EUR 225 |
Description: Available in various conjugation types. |
SUFU Antibody |
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E309471 | EnoGene | 200ul | EUR 275 |
Description: Available in various conjugation types. |
SUFU Antibody |
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35993 | SAB | 100ul | EUR 319 |
SUFU Antibody |
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35993-100ul | SAB | 100ul | EUR 302.4 |
SUFU antibody |
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70R-20639 | Fitzgerald | 50 ul | EUR 289 |
Description: Rabbit polyclonal SUFU antibody |
SUFU Antibody |
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ABD7687 | Lifescience Market | 100 ug | EUR 525.6 |
SUFU Antibody |
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ABD7828 | Lifescience Market | 100 ug | EUR 525.6 |
SUFU Antibody |
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1-CSB-PA022923GA01HU | Cusabio |
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Description: A polyclonal antibody against SUFU. Recognizes SUFU from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC |
SUFU Antibody |
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1-CSB-PA891560ESR2HU | Cusabio |
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Description: A polyclonal antibody against SUFU. Recognizes SUFU from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
SUFU Antibody |
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GWB-ML480C | GenWay Biotech | 50ug | Ask for price |
SUFU Antibody |
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GWB-ML481D | GenWay Biotech | 50ug | Ask for price |
SUFU Antibody |
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GWB-MN004D | GenWay Biotech | 50ug | Ask for price |
anti- SUFU antibody |
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FNab08373 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against SUFU |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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20-abx322260 | Abbexa |
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SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody |
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20-abx211712 | Abbexa |
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SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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20-abx127058 | Abbexa |
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SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx238373-100ug | Abbexa | 100 ug | EUR 610.8 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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20-abx141906 | Abbexa |
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SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx145714-100ug | Abbexa | 100 ug | EUR 469.2 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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20-abx005181 | Abbexa |
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SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx005181-100l | Abbexa | 100 µl | EUR 400 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx005181-20l | Abbexa | 20 µl | EUR 175 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx005181-50l | Abbexa | 50 µl | EUR 275 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx145714-1096tests | Abbexa | 10 × 96 tests | Ask for price |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx145714-596tests | Abbexa | 5 × 96 tests | Ask for price |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx145714-96tests | Abbexa | 96 tests | EUR 337.5 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx238373-100g | Abbexa | 100 µg | EUR 350 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx218817-100tests | Abbexa | 100 tests | Ask for price |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx218817-200tests | Abbexa | 200 tests | Ask for price |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx218817-20tests | Abbexa | 20 tests | EUR 350 |
SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody |
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abx211712-100l | Abbexa | 100 µl | EUR 350 |
SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody |
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abx211712-50l | Abbexa | 50 µl | EUR 250 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx322260-100g | Abbexa | 100 µg | EUR 350 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx322260-50g | Abbexa | 50 µg | EUR 237.5 |
SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody |
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abx433613-100g | Abbexa | 100 µg | EUR 387.5 |
OACA07036-50UL - SUFU Antibody |
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OACA07036-50UL | Aviva Systems Biology | 50ul | EUR 189 |
OAAN01995-50UL - SUFU Antibody |
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OAAN01995-50UL | Aviva Systems Biology | 50ul | EUR 149 |
OACA07036-100UL - SUFU Antibody |
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OACA07036-100UL | Aviva Systems Biology | 100ul | EUR 230 |
OAGA04566-100UL - SUFU Antibody |
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OAGA04566-100UL | Aviva Systems Biology | 100ul | EUR 369 |
OAAN01995-100UL - SUFU Antibody |
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OAAN01995-100UL | Aviva Systems Biology | 100ul | EUR 249 |
OAAN01995-200UL - SUFU Antibody |
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OAAN01995-200UL | Aviva Systems Biology | 200ul | EUR 389 |
OAAL00696-100UG - SUFU Antibody |
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OAAL00696-100UG | Aviva Systems Biology | 100ug | EUR 349 |
SUFU (pS342) Antibody |
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20-abx000504 | Abbexa |
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SUFU Polyclonal Antibody |
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E96757 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
SUFU Polyclonal Antibody |
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E913429 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
SUFU Conjugated Antibody |
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C35993 | SAB | 100ul | EUR 476.4 |
SUFU Conjugated Antibody |
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C39155 | SAB | 100ul | EUR 476.4 |
Suppressor of Fused (SUFU) Antibody |
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E8RT1600 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
Polyclonal SUFU Antibody (C-term) |
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APR04889G | Leading Biology | 0.1ml | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU (C-term). This antibody is tested and proven to work in the following applications: |
Polyclonal SUFU antibody - middle region |
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APR00655G | Leading Biology | 0.05mg | EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications: |
Polyclonal SUFU antibody - middle region |
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APR00749G | Leading Biology | 0.05mg | EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications: |
Monoclonal SUFU Antibody, Clone: 1783CT536.263.29 |
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AMM02579G | Leading Biology | 0.1ml | EUR 580.8 |
Description: A Monoclonal antibody against Human SUFU. The antibodies are raised in Mouse and are from clone 1783CT536.263.29. This antibody is applicable in WB, E |
Sufu (Phospho-Ser342) Antibody |
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11552 | SAB | 100ul | EUR 319 |
Sufu (Phospho-Ser342) Antibody |
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11552-100ul | SAB | 100ul | EUR 302.4 |
Sufu (Phospho-Ser342) Antibody |
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11552-50ul | SAB | 50ul | EUR 224.4 |
Sufu (Phospho-Ser342) Antibody |
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E011552 | EnoGene | 100μg/100μl | EUR 225 |
Description: Available in various conjugation types. |
SUFU (Phospho-Ser346) Antibody |
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SAB260P | SAB | 100ul | EUR 319 |
SUFU Mouse Monoclonal Antibody |
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E10G34348 | EnoGene | 100 μl | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
SUFU Rabbit Polyclonal Antibody |
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E10G00795 | EnoGene | 100 μl | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
Polyclonal SUFU antibody - N-terminal region |
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APR00623G | Leading Biology | 0.05mg | EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - N-terminal region. This antibody is tested and proven to work in the following applications: |
ARP33018_P050-25UL - SUFU Antibody - middle region |
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ARP33018_P050-25UL | Aviva Systems Biology | 25ul | EUR 99 |
ARP87204_P050-25UL - SUFU Antibody - middle region |
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ARP87204_P050-25UL | Aviva Systems Biology | 25ul | EUR 99 |
ARP87205_P050-25UL - SUFU Antibody - middle region |
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ARP87205_P050-25UL | Aviva Systems Biology | 25ul | EUR 99 |
ARP39233_P050-25UL - SUFU Antibody - middle region |
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ARP39233_P050-25UL | Aviva Systems Biology | 25ul | EUR 99 |
ARP33018_P050 - SUFU antibody - middle region (ARP33018_P050) |
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ARP33018_P050 | Aviva Systems Biology | 100ul | EUR 389 |
ARP87204_P050 - SUFU Antibody - middle region (ARP87204_P050) |
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ARP87204_P050 | Aviva Systems Biology | 100ul | EUR 389 |
ARP87205_P050 - SUFU Antibody - middle region (ARP87205_P050) |
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ARP87205_P050 | Aviva Systems Biology | 100ul | EUR 389 |
ARP39233_P050 - SUFU antibody - middle region (ARP39233_P050) |
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ARP39233_P050 | Aviva Systems Biology | 100ul | EUR 389 |
SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody |
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abx332917-100ul | Abbexa | 100 ul | EUR 560.4 |
SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody |
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abx332917-100g | Abbexa | 100 µg | Ask for price |
SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody |
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abx332917-20g | Abbexa | 20 µg | EUR 412.5 |
SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody |
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abx332917-50g | Abbexa | 50 µg | Ask for price |
Monoclonal SUFU Antibody (monoclonal) (M01), Clone: 1B2 |
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AMM04155G | Leading Biology | 0.1mg | EUR 580.8 |
Description: A Monoclonal antibody against Human SUFU (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B2. This antibody is applicable in E |
ARP33019_P050-25UL - SUFU Antibody - N-terminal region |
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ARP33019_P050-25UL | Aviva Systems Biology | 25ul | EUR 99 |
SUFU |
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E8ER1802-68 | EnoGene | 100ul | EUR 275 |
Description: Available in various conjugation types. |
SUFU |
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CSB-CL891560HU | Cusabio | 10 μg plasmid + 200μl Glycerol | Ask for price |
SUFU |
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pro-938 | ProSpec Tany | 5µg | EUR 60 |
Description: Recombinant Human Suppressor of Fused Homolog |
Sufu (Phospho-Ser342) Polyclonal Conjugated Antibody |
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C11552 | SAB | 100ul | EUR 476.4 |
SUFU siRNA |
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20-abx935622 | Abbexa |
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SUFU siRNA |
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20-abx935623 | Abbexa |
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ARP33019_P050 - SUFU |
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ARP33019_P050 | Aviva Systems Biology | 100ul | EUR 389 |
SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein |
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20-abx261500 | Abbexa |
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SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein |
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abx261500-100g | Abbexa | 100 µg | EUR 3550 |
SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein |
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abx261500-10g | Abbexa | 10 µg | EUR 325 |
SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein |
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abx261500-2g | Abbexa | 2 µg | EUR 225 |
Rabbit Anti-Human SUFU Polyclonal Antibody, Phospho-Ser342 |
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CPB-869RH | Creative Diagnostics | 100 ul | EUR 670.8 |
SUFU Mouse mAb |
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E2618296 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
SUFU Rabbit pAb |
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E2513429 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
SUFU Rabbit pAb |
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E2506757 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
SUFU Rabbit pAb |
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A13429-100ul | Abclonal | 100 ul | EUR 369.6 |
SUFU Rabbit pAb |
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A13429-200ul | Abclonal | 200 ul | EUR 550.8 |
SUFU Rabbit pAb |
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A13429-20ul | Abclonal | 20 ul | EUR 219.6 |
SUFU Rabbit pAb |
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A13429-50ul | Abclonal | 50 ul | EUR 267.6 |
SUFU Rabbit pAb |
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A19438-100ul | Abclonal | 100 ul | Ask for price |
SUFU Rabbit pAb |
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A19438-200ul | Abclonal | 200 ul | Ask for price |
SUFU Rabbit pAb |
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A19438-20ul | Abclonal | 20 ul | Ask for price |
SUFU Rabbit pAb |
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A19438-50ul | Abclonal | 50 ul | EUR 369.6 |
SUFU Rabbit pAb |
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A6757-100ul | Abclonal | 100 ul | EUR 369.6 |
SUFU Rabbit pAb |
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A6757-200ul | Abclonal | 200 ul | EUR 550.8 |
SUFU Rabbit pAb |
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A6757-20ul | Abclonal | 20 ul | EUR 219.6 |
SUFU Rabbit pAb |
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A6757-50ul | Abclonal | 50 ul | EUR 267.6 |
SUFU cloning plasmid |
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CSB-CL891560HU-10ug | Cusabio | 10ug | EUR 620.4 |
Description: A cloning plasmid for the SUFU gene. |
SUFU Blocking Peptide |
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DF7687-BP | Affbiotech | 1mg | EUR 234 |
Human SUFU Negative Regulator Of Hedgehog Signaling (SUFU) ELISA Kit |
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abx383558-96tests | Abbexa | 96 tests | EUR 1093.2 |
SUFU ELISA KIT|Human |
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EF003347 | Lifescience Market | 96 Tests | EUR 826.8 |
SUFU protein (His tag) |
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80R-1977 | Fitzgerald | 100 ug | EUR 329 |
Description: Recombinant human SUFU protein (His tag) |
Suppressor of Fused (SUFU) pSer342 rabbit polyclonal antibody, Aff - Purified |
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AP26040PU-N | Origene Technologies GmbH | 100 µg | Ask for price |
Suppressor of Fused (SUFU) pSer342 rabbit polyclonal antibody, Aff - Purified |
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AP26040PU-S | Origene Technologies GmbH | 50 µg | Ask for price |
Human SUFU shRNA Plasmid |
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20-abx959898 | Abbexa |
|
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Mouse SUFU shRNA Plasmid |
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20-abx973629 | Abbexa |
|
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Sufu (untagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), (10 ug) |
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RN210578 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU Recombinant Protein (Rat) |
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RP231659 | ABM | 100 ug | Ask for price |
SUFU Recombinant Protein (Mouse) |
|||
RP176351 | ABM | 100 ug | Ask for price |
SUFU Recombinant Protein (Mouse) |
|||
RP176354 | ABM | 100 ug | Ask for price |
SUFU Recombinant Protein (Human) |
|||
RP030583 | ABM | 100 ug | Ask for price |
SUFU (C-term) Rabbit pAb |
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E2619263 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
Sufu ORF Vector (Rat) (pORF) |
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ORF077221 | ABM | 1.0 ug DNA | EUR 607.2 |
SUFU (untagged)-Human suppressor of fused homolog (Drosophila) (SUFU) transcript variant 2 |
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SC328714 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU (untagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 1 |
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SC114403 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU ORF Vector (Human) (pORF) |
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ORF010195 | ABM | 1.0 ug DNA | EUR 114 |
Sufu ORF Vector (Mouse) (pORF) |
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ORF058785 | ABM | 1.0 ug DNA | EUR 607.2 |
Sufu ORF Vector (Mouse) (pORF) |
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ORF058786 | ABM | 1.0 ug DNA | EUR 607.2 |
Human SUFU knockout cell line |
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ABC-KH14885 | AcceGen | 1 vial | Ask for price |
Description: Human SUFU knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Sufu (Myc-DDK-tagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), (10 ug) |
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RR210578 | Origene Technologies GmbH | 10 µg | Ask for price |
Sufu (GFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2 |
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MG207758 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU (GFP-tagged) - Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 1 |
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RG201021 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU (GFP-tagged) - Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 2 |
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RG230076 | Origene Technologies GmbH | 10 µg | Ask for price |
Human SUFU knockdown cell line |
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ABC-KD14885 | AcceGen | 1 vial | Ask for price |
Description: Human SUFU knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human SUFU Protein Lysate 20ug |
|||
IHUSUFUPLLY20UG | Innovative research | each | EUR 211 |
Description: Human SUFU Protein Lysate 20ug |
Sufu (untagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2, (10ug) |
|||
MC216599 | Origene Technologies GmbH | 10 µg | Ask for price |
Sufu (untagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1, (10ug) |
|||
MC216658 | Origene Technologies GmbH | 10 µg | Ask for price |
OCOA02270-20UG - SUFU Protein Lysate |
|||
OCOA02270-20UG | Aviva Systems Biology | 20ug | EUR 169 |
Sufu (GFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu) transcript variant 1, (10ug) |
|||
MG222908 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU Over-expression Lysate Product |
|||
GWB-196C07 | GenWay Biotech | 0.1 mg | Ask for price |
SUFU (Myc-DDK-tagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 2 |
|||
RC230076 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU (Myc-DDK-tagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 1 |
|||
RC201021 | Origene Technologies GmbH | 10 µg | Ask for price |
Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1 |
|||
MR222908 | Origene Technologies GmbH | 10 µg | Ask for price |
Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2 |
|||
MR207758 | Origene Technologies GmbH | 10 µg | Ask for price |
Sufu sgRNA CRISPR Lentivector set (Rat) |
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K7545701 | ABM | 3 x 1.0 ug | EUR 406.8 |
Lenti ORF particles, Sufu (GFP-tagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), 200ul, >10^7 TU/mL |
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RR210578L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF clone of Sufu (mGFP-tagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), (10 ug) |
|||
RR210578L4 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU 3'UTR GFP Stable Cell Line |
|||
TU074900 | ABM | 1.0 ml | EUR 2799.6 |
Sufu 3'UTR GFP Stable Cell Line |
|||
TU271384 | ABM | 1.0 ml | Ask for price |
Sufu 3'UTR GFP Stable Cell Line |
|||
TU169919 | ABM | 1.0 ml | Ask for price |
SUFU sgRNA CRISPR Lentivector set (Human) |
|||
K2311501 | ABM | 3 x 1.0 ug | EUR 406.8 |
Sufu sgRNA CRISPR Lentivector set (Mouse) |
|||
K4415301 | ABM | 3 x 1.0 ug | EUR 406.8 |
Lenti ORF particles, Sufu (Myc-DDK-tagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), 200ul, >10^7 TU/mL |
|||
RR210578L3V | Origene Technologies GmbH | 200 µl | Ask for price |
SUFU Protein Vector (Rat) (pPM-C-HA) |
|||
PV308884 | ABM | 500 ng | EUR 723.6 |
AAP87204-100UG - SUFU Peptide - middle region |
|||
AAP87204-100UG | Aviva Systems Biology | 100ug | EUR 99 |
AAP87205-100UG - SUFU Peptide - middle region |
|||
AAP87205-100UG | Aviva Systems Biology | 100ug | EUR 99 |
AAP39233-100UG - SUFU Peptide - middle region |
|||
AAP39233-100UG | Aviva Systems Biology | 100ug | EUR 99 |
AAP33018-100UG - SUFU Peptide - middle region |
|||
AAP33018-100UG | Aviva Systems Biology | 100ug | EUR 99 |
Lenti ORF clone of Sufu (Myc-DDK-tagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), (10 ug) |
|||
RR210578L3 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU Protein Vector (Rat) (pPB-C-His) |
|||
PV308882 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Rat) (pPB-N-His) |
|||
PV308883 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Rat) (pPM-C-His) |
|||
PV308885 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Human) (pPM-C-HA) |
|||
PV040779 | ABM | 500 ng | EUR 394.8 |
SUFU Protein Vector (Mouse) (pPM-C-HA) |
|||
PV235140 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPM-C-HA) |
|||
PV235144 | ABM | 500 ng | EUR 723.6 |
Lenti ORF clone of Sufu (mGFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1 |
|||
MR222908L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Sufu (mGFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2 |
|||
MR207758L4 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU Protein Vector (Human) (pPB-C-His) |
|||
PV040777 | ABM | 500 ng | EUR 394.8 |
SUFU Protein Vector (Human) (pPB-N-His) |
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PV040778 | ABM | 500 ng | EUR 394.8 |
SUFU Protein Vector (Human) (pPM-C-His) |
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PV040780 | ABM | 500 ng | EUR 394.8 |
SUFU Protein Vector (Mouse) (pPB-C-His) |
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PV235138 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPB-N-His) |
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PV235139 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPM-C-His) |
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PV235141 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPB-C-His) |
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PV235142 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPB-N-His) |
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PV235143 | ABM | 500 ng | EUR 723.6 |
SUFU Protein Vector (Mouse) (pPM-C-His) |
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PV235145 | ABM | 500 ng | EUR 723.6 |
Sufu 3'UTR Luciferase Stable Cell Line |
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TU119919 | ABM | 1.0 ml | Ask for price |
SUFU 3'UTR Luciferase Stable Cell Line |
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TU024900 | ABM | 1.0 ml | EUR 2799.6 |
Sufu 3'UTR Luciferase Stable Cell Line |
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TU221384 | ABM | 1.0 ml | Ask for price |
Lenti-ORF clone of SUFU (mGFP-tagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 2 |
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RC230076L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Sufu sgRNA CRISPR Lentivector (Rat) (Target 1) |
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K7545702 | ABM | 1.0 ug DNA | EUR 184.8 |
Sufu sgRNA CRISPR Lentivector (Rat) (Target 2) |
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K7545703 | ABM | 1.0 ug DNA | EUR 184.8 |
Sufu sgRNA CRISPR Lentivector (Rat) (Target 3) |
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K7545704 | ABM | 1.0 ug DNA | EUR 184.8 |
Lenti-ORF clone of SUFU (Myc-DDK-tagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 2 |
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RC230076L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1 |
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MR222908L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2 |
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MR207758L3 | Origene Technologies GmbH | 10 µg | Ask for price |
SUFU sgRNA CRISPR Lentivector (Human) (Target 1) |
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K2311502 | ABM | 1.0 ug DNA | EUR 184.8 |
Conduction Band Power-Degree Engineering for Bettering Open-Circuit Voltage in Antimony Selenide Nanorod Array Photo voltaic Cells
Antimony selenide (Sb2 Se3 ) nanorod arrays alongside the [001] orientation are identified to switch photogenerated carriers quickly as a result of strongly anisotropic one-dimensional crystal construction. With superior light-trapping constructions, the Sb2 Se3 nanorod array-based photo voltaic cells have glorious broad spectral response properties, and better short-circuit present density than the traditional planar structured skinny movie photo voltaic cells. Nevertheless, the interface engineering for the Sb2 Se3 nanorod array-based photo voltaic cell is extra essential to extend the efficiency, as a result of it’s difficult to coat a compact buffer layer with good protection to type a uniform heterojunction interface attributable to its massive floor space and length-diameter ratio.
On this work, an intermeshing In2 S3 nanosheet-CdS composite because the buffer layer, compactly coating on the Sb2 Se3 nanorod floor is constructed. The appliance of In2 S3 -CdS composite buffers construct a gradient conduction band power configuration within the Sb2 Se3 /buffer heterojunction interface, which reduces the interface recombination and enhances the switch and assortment of photogenerated electrons. The energy-level regulation minimizes the open-circuit voltage deficit on the interfaces of buffer/Sb2 Se3 and buffer/ZnO layers within the Sb2 Se3 photo voltaic cells. Consequently, the Sb2 Se3 nanorod array photo voltaic cell primarily based on In2 S3 -CdS composite buffers achieves an effectivity of as excessive as 9.19% with a VOC of 461 mV.
Distinguishing between Isomeric Neoxanthin and Violaxanthin Esters in Yellow Flower Petals utilizing Liquid Chromatography-Photodiode Array Atmospheric Stress Chemical Ionization Mass Spectrometry and MS/MS
Rationale: Liquid chromatography-photodiode array atmospheric strain chemical ionization mass spectrometry (LC-PDA-APCI-MS) is used for the evaluation of assorted carotenoid pigments in crops. Amongst them, it’s tough to differentiate between the isomeric violaxanthin/neoxanthin esters.
Strategies: The yellow pigments of tomato petals have been extracted with acetone, and the extracts have been saved at -30°C to settle out the contaminating triacylglycerols bodily. The supernatants have been analyzed utilizing LC-PDA-APCI-MS with a high-resolution orbitrap MS for his or her precise lots. The anticipated carotenoid esters have been calculated with the mixture of carotenoids and fatty acids, they usually have been matched with the experimental precise lots. The fatty acid constructions within the carotenoid esters have been additionally recognized utilizing collision-induced dissociation (CID) MS/MS. The isomeric violaxanthin/neoxanthin esters have been distinguished utilizing CID MS/MS from their in-source dehydrated product ions as pseudoprecursor ions.
Outcomes: The in-source dehydrated ions [M-H2 O+H]+ of neoxanthin diesters predominated over their protonated molecules [M+H]+ within the LC-MS. Against this, the protonated molecules of violaxanthin diesters predominated. The 92 u loss product ions [M-H2 O-C7 H8 +H]+ have been noticed from the dehydrated violaxanthin diesters, however they weren’t generated from the dehydrated neoxanthin diesters within the CID MS/MS of their dehydrated pseudoprecursor ion [M-H2 O+H]+ .
Conclusions: The allene allyl carbocation in neoxanthin diesters was generated from dehydration after preferential protonation on the hydroxy group. The epoxide group of violaxanthin diesters opens simply after protonation; nonetheless, the dehydration didn’t proceed at this stage. The 92 u lack of C7 H8 was defined by the intramolecular [2+2] cycloaddition, which proceeded preferentially in dehydrated violaxanthin diesters as a result of the carbocations within the dehydrated species have been conjugated to the polyene and people double bonds have been depolarized within the CID MS/MS. Due to this fact, the isomeric neoxanthin/violaxanthin diesters have been distinguished utilizing LC-PDA-APCI-MS and MS/MS. This technique was a sensible and helpful technique of profiling the carotenoid esters of the yellow petals.
Beam pen lithography as a brand new software for spatially managed photochemistry, and its utilization within the synthesis of multivalent glycan arrays
Herein, we describe how cantilever-free scanning probes can be utilized to deposit precursor materials and subsequently irradiate the precursor to provoke polymerization, leading to a 3D lithographic technique whereby the place, peak and diameter of every function may be tuned independently. Particularly, acrylate and methacrylate monomers have been patterned onto thiol terminated glass and subsequently uncovered to UV mild produced brush polymers by a photoinduced radical acrylate polymerization response. Right here, we report the primary examples of glycan arrays, comprised of methacrylate brush polymers which are side-chain functionalized with α-glucose, by this new lithographic method.
Their binding with fluorophore labeled concanavalin A (ConA) was assayed by fluorescence microscopy. The fluorescence of those brush polymers was in comparison with glycan arrays composed of monolayers of α-mannosides and α-glucosides ready by combining polymer pen lithography (PPL) with the thiol-ene photochemical response or the copper-catalyzed azide-alkyne cycloaddition. At excessive ConA focus, the fluorescence sign of the brush polymer was practically 20 occasions better than that of the glycan monolayers, and the comb polymer arrays had a detection restrict practically two orders of magnitude higher than their monolayer counterparts.
Due to the potential of this technique to manage exactly the polymer size, the connection between restrict of detection and multivalency could possibly be explored, and it was discovered that the longer polymers (136 nm) are an order of magnitude extra delicate in the direction of ConA binding than the shorter polymers (Eight nm) and that binding affinity decreased systematically with size. These glycan arrays are a brand new software to check the function of multivalency on carbohydrate recognition, and the photopolymerization route in the direction of forming multivalent glycan scaffolds described herein, is a promising path to create multiplexed glycan arrays with nanoscale function dimensions.