Mixture of Antibody Arrays to Functionally Characterize Darkish Proteins in Human Olfactory Neuroepithelial Cells

The completion and annotation of the human proteome require the provision of knowledge associated to protein perform. Presently, greater than 1800 human genes represent the “darkish proteome,” which embody lacking proteins, uncharacterized human genes validated at protein degree, smORFs, proteins from lncRNAs, or any uncharacterized transcripts. Over the past years, totally different experimental workflows primarily based on multi-omics analyses, bioinformatics, and in vitro and in vivo research have been promoted by the Human Proteome Challenge Consortium to boost the annotation of darkish proteins.

On this chapter, we describe a technique that makes use of recombinant proteins and antibody arrays to ascertain an easy methodology in an effort to quickly characterize potential practical options of darkish proteins related to intracellular signaling dynamics and extracellular immune response in human cell cultures. Additional validating the strategy, this workflow was utilized to probe adjustments within the activation patterns of kinases and transcription components in addition to in cytokine manufacturing modulated by the darkish C1orf128 (PITHD1) protein in human olfactory neuroepithelial cells.

Identification of Antibody Biomarker Utilizing Excessive-Density Nucleic Acid Programmable Protein Array

A novel protein microarray expertise, known as high-density nucleic acid programmable protein array (HD-NAPPA), allows the serological screening of hundreds of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a standard glass microscope slide. By comparability, HD-NAPPA shows proteins in over 10,000 nanowells etched in a silicon slide.

Proteins on HD-NAPPA are expressed within the particular person remoted nanowells, through in vitro transcription and translation (IVTT), with none diffusion throughout incubation. Right here we describe the strategy for antibody biomarker identification utilizing HD-NAPPA, together with 4 major steps: (1) HD-NAPPA array protein expression, (2) main antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) picture scanning and information processing.

SUFU Antibody
E309471	EnoGene	200ul	EUR 275
Description: Available in various conjugation types.

SUFU Antibody
DF7687	Affinity Biosciences	100ul	EUR 280
Description: Human

SUFU Antibody
DF7687-100ul	Affinity Biosciences	100ul	EUR 280

SUFU Antibody
DF7687-200ul	Affinity Biosciences	200ul	EUR 350

SUFU Antibody
E035993	EnoGene	100μg/100μl	EUR 255
Description: Available in various conjugation types.

SUFU Antibody
E19-7687-1	EnoGene	50ug/50ul	EUR 145
Description: Available in various conjugation types.

SUFU Antibody
E19-7687-2	EnoGene	100ug/100ul	EUR 225
Description: Available in various conjugation types.

SUFU antibody
E39-08373	EnoGene	100ug/100ul	EUR 225
Description: Available in various conjugation types.

SUFU antibody
E39-10794	EnoGene	100ug/100ul	EUR 225
Description: Available in various conjugation types.

anti- SUFU antibody
FNab08373	FN Test	100µg	EUR 658.5
Description: Antibody raised against SUFU

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx238373-100ug	Abbexa	100 ug	EUR 610.8

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody
20-abx211712	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
20-abx322260	Abbexa	EUR 360.00 EUR 292.80	100 ul 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
20-abx127058	Abbexa	EUR 493.20 EUR 710.40	100 ul 200 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
20-abx005181	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
20-abx141906	Abbexa	EUR 444.00 EUR 727.20 EUR 360.00	100 ul 200 ul 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx145714-100ug	Abbexa	100 ug	EUR 469.2

SUFU (pS342) Antibody
20-abx000504	Abbexa	EUR 594.00 EUR 844.80 EUR 427.20	100 ul 200 ul 50 ul

SUFU Conjugated Antibody
C39155	SAB	100ul	EUR 476.4

SUFU Conjugated Antibody
C35993	SAB	100ul	EUR 476.4

SUFU Polyclonal Antibody
E913429	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

SUFU Polyclonal Antibody
E96757	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

Polyclonal SUFU Antibody (C-term)
APR04889G	Leading Biology	0.1ml	EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU (C-term). This antibody is tested and proven to work in the following applications:

Suppressor of Fused (SUFU) Antibody
E8RT1600	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

Monoclonal SUFU Antibody, Clone: 1783CT536.263.29
AMM02579G	Leading Biology	0.1ml	EUR 580.8
Description: A Monoclonal antibody against Human SUFU. The antibodies are raised in Mouse and are from clone 1783CT536.263.29. This antibody is applicable in WB, E

Polyclonal SUFU antibody - middle region
APR00655G	Leading Biology	0.05mg	EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications:

Polyclonal SUFU antibody - middle region
APR00749G	Leading Biology	0.05mg	EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications:

Sufu (Phospho-Ser342) Antibody
11552-100ul	SAB	100ul	EUR 302.4

Sufu (Phospho-Ser342) Antibody
11552-50ul	SAB	50ul	EUR 224.4

Sufu (Phospho-Ser342) Antibody
E011552	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

Polyclonal SUFU antibody - N-terminal region
APR00623G	Leading Biology	0.05mg	EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - N-terminal region. This antibody is tested and proven to work in the following applications:

SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody
abx332917-100ul	Abbexa	100 ul	EUR 560.4

Monoclonal SUFU Antibody (monoclonal) (M01), Clone: 1B2
AMM04155G	Leading Biology	0.1mg	EUR 580.8
Description: A Monoclonal antibody against Human SUFU (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B2. This antibody is applicable in E

SUFU
E8ER1802-68	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

SUFU
pro-938	ProSpec Tany	5µg	EUR 60
Description: Recombinant Human Suppressor of Fused Homolog

SUFU siRNA
20-abx935622	Abbexa	EUR 661.20 EUR 878.40	15 nmol 30 nmol

SUFU siRNA
20-abx935623	Abbexa	EUR 661.20 EUR 878.40	15 nmol 30 nmol

Sufu (Phospho-Ser342) Polyclonal Conjugated Antibody
C11552	SAB	100ul	EUR 476.4

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein
20-abx261500	Abbexa	EUR 4101.60 EUR 393.60 EUR 276.00	1 mg 20 ug 5 ug

SUFU Mouse mAb
E2618296	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

SUFU Rabbit pAb
A13429-100ul	Abclonal	100 ul	EUR 369.6

SUFU Rabbit pAb
A13429-200ul	Abclonal	200 ul	EUR 550.8

SUFU Rabbit pAb
A13429-20ul	Abclonal	20 ul	EUR 219.6

SUFU Rabbit pAb
A13429-50ul	Abclonal	50 ul	EUR 267.6

SUFU Rabbit pAb
A19438-100ul	Abclonal	100 ul	Ask for price

SUFU Rabbit pAb
A19438-200ul	Abclonal	200 ul	Ask for price

SUFU Rabbit pAb
A19438-20ul	Abclonal	20 ul	Ask for price

SUFU Rabbit pAb
A19438-50ul	Abclonal	50 ul	EUR 369.6

SUFU Rabbit pAb
A6757-100ul	Abclonal	100 ul	EUR 369.6

SUFU Rabbit pAb
A6757-200ul	Abclonal	200 ul	EUR 550.8

SUFU Rabbit pAb
A6757-20ul	Abclonal	20 ul	EUR 219.6

SUFU Rabbit pAb
A6757-50ul	Abclonal	50 ul	EUR 267.6

SUFU Rabbit pAb
E2506757	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

SUFU Rabbit pAb
E2513429	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

Rabbit Anti-Human SUFU Polyclonal Antibody, Phospho-Ser342
CPB-869RH	Creative Diagnostics	100 ul	EUR 670.8

SUFU cloning plasmid
CSB-CL891560HU-10ug	Cusabio	10ug	EUR 620.4
Description: A cloning plasmid for the SUFU gene.

SUFU Blocking Peptide
DF7687-BP	Affbiotech	1mg	EUR 234

Human SUFU Negative Regulator Of Hedgehog Signaling (SUFU) ELISA Kit
abx383558-96tests	Abbexa	96 tests	EUR 1093.2

SUFU protein (His tag)
80R-1977	Fitzgerald	100 ug	EUR 386.4
Description: Recombinant human SUFU protein (His tag)

SUFU ELISA KIT|Human
EF003347	Lifescience Market	96 Tests	EUR 826.8

Human SUFU shRNA Plasmid
20-abx959898	Abbexa	EUR 961.20 EUR 1345.20	150 µg 300 µg

Mouse SUFU shRNA Plasmid
20-abx973629	Abbexa	EUR 961.20 EUR 1345.20	150 µg 300 µg

SUFU Recombinant Protein (Rat)
RP231659	ABM	100 ug	Ask for price

SUFU Recombinant Protein (Mouse)
RP176351	ABM	100 ug	Ask for price

SUFU Recombinant Protein (Mouse)
RP176354	ABM	100 ug	Ask for price

SUFU Recombinant Protein (Human)
RP030583	ABM	100 ug	Ask for price

Sufu ORF Vector (Rat) (pORF)
ORF077221	ABM	1.0 ug DNA	EUR 607.2

SUFU (C-term) Rabbit pAb
E2619263	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

SUFU ORF Vector (Human) (pORF)
ORF010195	ABM	1.0 ug DNA	EUR 114

Sufu ORF Vector (Mouse) (pORF)
ORF058785	ABM	1.0 ug DNA	EUR 607.2

Sufu ORF Vector (Mouse) (pORF)
ORF058786	ABM	1.0 ug DNA	EUR 607.2

Human SUFU Protein Lysate 20ug
IHUSUFUPLLY20UG	Innovative research	each	EUR 211
Description: Human SUFU Protein Lysate 20ug

Sufu sgRNA CRISPR Lentivector set (Rat)
K7545701	ABM	3 x 1.0 ug	EUR 406.8

Sufu 3'UTR GFP Stable Cell Line
TU271384	ABM	1.0 ml	Ask for price

SUFU 3'UTR GFP Stable Cell Line
TU074900	ABM	1.0 ml	EUR 2799.6

Sufu 3'UTR GFP Stable Cell Line
TU169919	ABM	1.0 ml	Ask for price

SUFU sgRNA CRISPR Lentivector set (Human)
K2311501	ABM	3 x 1.0 ug	EUR 406.8

Sufu sgRNA CRISPR Lentivector set (Mouse)
K4415301	ABM	3 x 1.0 ug	EUR 406.8

SUFU Protein Vector (Rat) (pPM-C-HA)
PV308884	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Rat) (pPB-C-His)
PV308882	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Rat) (pPB-N-His)
PV308883	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Rat) (pPM-C-His)
PV308885	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Human) (pPM-C-HA)
PV040779	ABM	500 ng	EUR 394.8

SUFU Protein Vector (Mouse) (pPM-C-HA)
PV235140	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Mouse) (pPM-C-HA)
PV235144	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Human) (pPB-C-His)
PV040777	ABM	500 ng	EUR 394.8

SUFU Protein Vector (Human) (pPB-N-His)
PV040778	ABM	500 ng	EUR 394.8

SUFU Protein Vector (Human) (pPM-C-His)
PV040780	ABM	500 ng	EUR 394.8

SUFU Protein Vector (Mouse) (pPB-C-His)
PV235138	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Mouse) (pPB-N-His)
PV235139	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Mouse) (pPM-C-His)
PV235141	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Mouse) (pPB-C-His)
PV235142	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Mouse) (pPB-N-His)
PV235143	ABM	500 ng	EUR 723.6

SUFU Protein Vector (Mouse) (pPM-C-His)
PV235145	ABM	500 ng	EUR 723.6

Sufu 3'UTR Luciferase Stable Cell Line
TU221384	ABM	1.0 ml	Ask for price

Sufu 3'UTR Luciferase Stable Cell Line
TU119919	ABM	1.0 ml	Ask for price

SUFU 3'UTR Luciferase Stable Cell Line
TU024900	ABM	1.0 ml	EUR 2799.6

Sufu sgRNA CRISPR Lentivector (Rat) (Target 1)
K7545702	ABM	1.0 ug DNA	EUR 184.8

Sufu sgRNA CRISPR Lentivector (Rat) (Target 2)
K7545703	ABM	1.0 ug DNA	EUR 184.8

Sufu sgRNA CRISPR Lentivector (Rat) (Target 3)
K7545704	ABM	1.0 ug DNA	EUR 184.8

SUFU sgRNA CRISPR Lentivector (Human) (Target 1)
K2311502	ABM	1.0 ug DNA	EUR 184.8

SUFU sgRNA CRISPR Lentivector (Human) (Target 2)
K2311503	ABM	1.0 ug DNA	EUR 184.8

SUFU sgRNA CRISPR Lentivector (Human) (Target 3)
K2311504	ABM	1.0 ug DNA	EUR 184.8

Sufu sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4415302	ABM	1.0 ug DNA	EUR 184.8

Sufu sgRNA CRISPR Lentivector (Mouse) (Target 2)
K4415303	ABM	1.0 ug DNA	EUR 184.8

Sufu sgRNA CRISPR Lentivector (Mouse) (Target 3)
K4415304	ABM	1.0 ug DNA	EUR 184.8

Recombinant Human SUFU Protein, His, E.coli-1mg
QP13639-1mg	EnQuireBio	1mg	EUR 3308.4

Recombinant Human SUFU Protein, His, E.coli-5ug
QP13639-5ug	EnQuireBio	5ug	EUR 186

Recombinant Human SUFU Protein, His, E.coli-20ug
QP13639-20ug	EnQuireBio	20ug	EUR 241.2

Anti-SUFU Antibody
A02279-1	BosterBio	100ug/vial	EUR 352.8

Anti-SUFU antibody
PAab08373	Lifescience Market	100 ug	EUR 463.2

Anti-SUFU Antibody
ER1802-68	HUABIO	100ul	EUR 189
Description: Su(fu) (for Suppressor-of-Fused) is a key negative regulator in the vertebrate Hedgehog signaling pathway. Su(fu) interacts with genes encoding proteins in this signal transduction pathway. In Drosophila, Intracellular transduction of the Hedgehog pathway involves the release of a large complex containing Su(fu). Su(fu) inhibits the activity of the transcription factor Gli1 and interacts with Gli2, Gli3 and the serine/threonine kinase Fused. Su(fu) is widely expressed in adult and embryonic tissues with higher expression in tissues patterned by hegdehog signaling. The Su(fu) gene locus maps to a region that is deleted in glioblastomas, prostate cancer, malignant melanoma and endometrial cancer.

Anti-SUFU antibody
STJ28840	St John's Laboratory	100 µl	EUR 332.4
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Anti-SUFU antibody
STJ11100632	St John's Laboratory	50 µl	EUR 344.4
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Anti-SUFU antibody
STJ115390	St John's Laboratory	100 µl	EUR 332.4
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Mouse Suppressor of fused homolog, Sufu ELISA KIT
ELI-29930m	Lifescience Market	96 Tests	EUR 1038

Human Suppressor of fused homolog, SUFU ELISA KIT
ELI-18767h	Lifescience Market	96 Tests	EUR 988.8

SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV653185	ABM	1.0 ug DNA	EUR 818.4

SUFU Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV653189	ABM	1.0 ug DNA	EUR 818.4

SUFU Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV653190	ABM	1.0 ug DNA	EUR 818.4

SUFU Suppressor of Fused Homolog Human Recombinant Protein
PROTQ9UMX1	BosterBio	Regular: 20ug	EUR 380.4
Description: SUFU Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 504 amino acids (1-484 a.a.) and having a molecular mass of 56.1kDa.;SUFU is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Rabbit anti-SUFU Antibody
DL96331A-100ul	DL Develop	100 ul	EUR 334
Description: Suppressor of fused homolog; SUFUH

Rabbit anti-SUFU Antibody
DL96331A-50ul	DL Develop	50 ul	EUR 233.8
Description: Suppressor of fused homolog; SUFUH

Rabbit anti-SUFU Antibody
YLD7492-100ul	Shanghai YL Biotech	100 ul	EUR 320
Description: Rabbit polyclonal antibody to SUFU

Rabbit anti-SUFU Antibody
YLD7492-50ul	Shanghai YL Biotech	50 ul	EUR 200
Description: Rabbit polyclonal antibody to SUFU

Phospho-SUFU (Ser342) Antibody
CSB-PA229368-	Cusabio	each	EUR 402
Description: A polyclonal antibody against Phospho-SUFU (Ser342). Recognizes Phospho-SUFU (Ser342) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

Phospho-SUFU (Ser342) Antibody
CSB-PA229368-100ul	Cusabio	100ul	EUR 434.4
Description: A polyclonal antibody against Phospho-SUFU (Ser342). Recognizes Phospho-SUFU (Ser342) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K7545705	ABM	3 x 1.0 ug	EUR 451.2

SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K2311505	ABM	3 x 1.0 ug	EUR 451.2

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K4415305	ABM	3 x 1.0 ug	EUR 451.2

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)
K7545706	ABM	1.0 ug DNA	EUR 200.4

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)
K7545707	ABM	1.0 ug DNA	EUR 200.4

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)
K7545708	ABM	1.0 ug DNA	EUR 200.4

Anti-Phospho-SUFU-(S342) antibody
STJ22424	St John's Laboratory	100 µl	EUR 471.6
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K2311506	ABM	1.0 ug DNA	EUR 200.4

SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K2311507	ABM	1.0 ug DNA	EUR 200.4

SUFU sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K2311508	ABM	1.0 ug DNA	EUR 200.4

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K4415306	ABM	1.0 ug DNA	EUR 200.4

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K4415307	ABM	1.0 ug DNA	EUR 200.4

Sufu sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K4415308	ABM	1.0 ug DNA	EUR 200.4

SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV653186	ABM	1.0 ug DNA	EUR 818.4

SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV653187	ABM	1.0 ug DNA	EUR 888

SUFU Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV653188	ABM	1.0 ug DNA	EUR 888

Rabbit Anti SUFU Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot,Immunofluorescence)
IRBAMLSUFUAP632120UL	Innovative research	each	EUR 346
Description: Rabbit Anti SUFU Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot,Immunofluorescence)

Rabbit Anti SUFU Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot,Immunofluorescence)
IRBAMLSUFUAP632200UL	Innovative research	each	EUR 509
Description: Rabbit Anti SUFU Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot,Immunofluorescence)

Sufugolix
HY-100209	MedChemExpress	100mg	EUR 1614

Phospho-SUFU-S342 Rabbit pAb
AP0457-100ul	Abclonal	100 ul	EUR 460.8

Phospho-SUFU-S342 Rabbit pAb
AP0457-200ul	Abclonal	200 ul	EUR 664.8

Phospho-SUFU-S342 Rabbit pAb
AP0457-20ul	Abclonal	20 ul	Ask for price

Phospho-SUFU-S342 Rabbit pAb
AP0457-50ul	Abclonal	50 ul	EUR 318

Antibody/Protein conjugation to FITC kit (sufficient for 2 reactions)
80230	Alpha Diagnostics	1 kit	EUR 534

Ferret Primary Antibody (IgA) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80188-A	Alpha Diagnostics	1 kit	EUR 781.2

Ferret Primary Antibody (IgG) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80188-G	Alpha Diagnostics	1 kit	EUR 781.2

Ferret Primary Antibody (IgM) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80188-M	Alpha Diagnostics	1 kit	EUR 781.2

mPEG antibody purification kit (mPEG based affinity matrix; sufficient to purify 10-20 mg of antibody from immunized animals) 10 ml column
PEG-AFP-10A	Alpha Diagnostics	1 Kit	EUR 927.6

Hamster Serum Antibody detection ELISA kit, Qualitative (sufficient for 500-1000 tests)
80180	Alpha Diagnostics	1 kit	EUR 781.2

Goat Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80165	Alpha Diagnostics	1 Kit	EUR 781.2

Pig Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80186	Alpha Diagnostics	1 kit	EUR 781.2

Human Primary Antibody (IgA) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80170-A	Alpha Diagnostics	1 kit	EUR 781.2

Human Primary Antibody (IgE) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80170-E	Alpha Diagnostics	1 kit	EUR 781.2

G. Pig Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80171	Alpha Diagnostics	1 Kit	EUR 781.2

Sheep Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80166	Alpha Diagnostics	1 Kit	EUR 781.2

Human Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80170	Alpha Diagnostics	1 Kit	EUR 781.2

Donkey Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80172	Alpha Diagnostics	1 Kit	EUR 781.2

Horse Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80173	Alpha Diagnostics	1 Kit	EUR 781.2

Monkey Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80175	Alpha Diagnostics	1 Kit	EUR 781.2

Bovine Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80185	Alpha Diagnostics	1 kit	EUR 781.2

Turkey Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80187	Alpha Diagnostics	1 kit	EUR 781.2

Ferret Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80188	Alpha Diagnostics	1 kit	EUR 781.2

Human Primary Antibody (IgG1) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80170-G1	Alpha Diagnostics	1 kit	EUR 781.2

Human Primary Antibody (IgG2) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80170-G2	Alpha Diagnostics	1 kit	EUR 781.2

Chicken Primary Antibody (A+G+M) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80176	Alpha Diagnostics	1 kit	EUR 781.2

mPEG protein purification kit (Antibody based affinity matrix; sufficient to purify 2-4 mg protein) 2 ml column
PEG-AFP-02P	Alpha Diagnostics	1 Kit	EUR 416.4

Chicken IgY Antibody (A+G+M) detection and titration in whole egg yolks ELISA kit, Qualitative (sufficient for 500-1000 tests)
80177	Alpha Diagnostics	1 kit	EUR 781.2

Mouse Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-A	Alpha Diagnostics	1 kit	EUR 781.2

Mouse Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-E	Alpha Diagnostics	1 kit	EUR 781.2

Rat Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155-A	Alpha Diagnostics	1 kit	EUR 781.2

Rat Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155-E	Alpha Diagnostics	1 kit	EUR 781.2

Mouse Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150	Alpha Diagnostics	1 Kit	EUR 781.2

Rat Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155	Alpha Diagnostics	1 Kit	EUR 781.2

Rabbit Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80160	Alpha Diagnostics	1 Kit	EUR 781.2

Mouse Primary Antibody (IgG1) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-G1	Alpha Diagnostics	1 kit	EUR 781.2

Mouse Primary Antibody (IgG2a) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-G2a	Alpha Diagnostics	1 kit	EUR 781.2

Conduction Band Power-Degree Engineering for Bettering Open-Circuit Voltage in Antimony Selenide Nanorod Array Photo voltaic Cells

Antimony selenide (Sb₂ Se₃ ) nanorod arrays alongside the [001] orientation are identified to switch photogenerated carriers quickly as a result of strongly anisotropic one-dimensional crystal construction. With superior light-trapping constructions, the Sb₂ Se₃ nanorod array-based photo voltaic cells have glorious broad spectral response properties, and better short-circuit present density than the traditional planar structured skinny movie photo voltaic cells. Nevertheless, the interface engineering for the Sb₂ Se₃ nanorod array-based photo voltaic cell is extra essential to extend the efficiency, as a result of it’s difficult to coat a compact buffer layer with good protection to type a uniform heterojunction interface attributable to its massive floor space and length-diameter ratio.

On this work, an intermeshing In₂ S₃ nanosheet-CdS composite because the buffer layer, compactly coating on the Sb₂ Se₃ nanorod floor is constructed. The appliance of In₂ S₃ -CdS composite buffers construct a gradient conduction band power configuration within the Sb₂ Se₃ /buffer heterojunction interface, which reduces the interface recombination and enhances the switch and assortment of photogenerated electrons. The energy-level regulation minimizes the open-circuit voltage deficit on the interfaces of buffer/Sb₂ Se₃ and buffer/ZnO layers within the Sb₂ Se₃ photo voltaic cells. Consequently, the Sb₂ Se₃ nanorod array photo voltaic cell primarily based on In₂ S₃ -CdS composite buffers achieves an effectivity of as excessive as 9.19% with a V_OC of 461 mV.

Distinguishing between Isomeric Neoxanthin and Violaxanthin Esters in Yellow Flower Petals utilizing Liquid Chromatography-Photodiode Array Atmospheric Stress Chemical Ionization Mass Spectrometry and MS/MS

Rationale: Liquid chromatography-photodiode array atmospheric strain chemical ionization mass spectrometry (LC-PDA-APCI-MS) is used for the evaluation of assorted carotenoid pigments in crops. Amongst them, it’s tough to differentiate between the isomeric violaxanthin/neoxanthin esters.

Strategies: The yellow pigments of tomato petals have been extracted with acetone, and the extracts have been saved at -30°C to settle out the contaminating triacylglycerols bodily. The supernatants have been analyzed utilizing LC-PDA-APCI-MS with a high-resolution orbitrap MS for his or her precise lots. The anticipated carotenoid esters have been calculated with the mixture of carotenoids and fatty acids, they usually have been matched with the experimental precise lots. The fatty acid constructions within the carotenoid esters have been additionally recognized utilizing collision-induced dissociation (CID) MS/MS. The isomeric violaxanthin/neoxanthin esters have been distinguished utilizing CID MS/MS from their in-source dehydrated product ions as pseudoprecursor ions.

Outcomes: The in-source dehydrated ions [M-H₂ O+H]⁺ of neoxanthin diesters predominated over their protonated molecules [M+H]⁺ within the LC-MS. Against this, the protonated molecules of violaxanthin diesters predominated. The 92 u loss product ions [M-H₂ O-C₇ H₈ +H]⁺ have been noticed from the dehydrated violaxanthin diesters, however they weren’t generated from the dehydrated neoxanthin diesters within the CID MS/MS of their dehydrated pseudoprecursor ion [M-H₂ O+H]⁺ .

Conclusions: The allene allyl carbocation in neoxanthin diesters was generated from dehydration after preferential protonation on the hydroxy group. The epoxide group of violaxanthin diesters opens simply after protonation; nonetheless, the dehydration didn’t proceed at this stage. The 92 u lack of C₇ H₈ was defined by the intramolecular [2+2] cycloaddition, which proceeded preferentially in dehydrated violaxanthin diesters as a result of the carbocations within the dehydrated species have been conjugated to the polyene and people double bonds have been depolarized within the CID MS/MS. Due to this fact, the isomeric neoxanthin/violaxanthin diesters have been distinguished utilizing LC-PDA-APCI-MS and MS/MS. This technique was a sensible and helpful technique of profiling the carotenoid esters of the yellow petals.

Beam pen lithography as a brand new software for spatially managed photochemistry, and its utilization within the synthesis of multivalent glycan arrays

Herein, we describe how cantilever-free scanning probes can be utilized to deposit precursor materials and subsequently irradiate the precursor to provoke polymerization, leading to a 3D lithographic technique whereby the place, peak and diameter of every function may be tuned independently. Particularly, acrylate and methacrylate monomers have been patterned onto thiol terminated glass and subsequently uncovered to UV mild produced brush polymers by a photoinduced radical acrylate polymerization response. Right here, we report the primary examples of glycan arrays, comprised of methacrylate brush polymers which are side-chain functionalized with α-glucose, by this new lithographic method.

Their binding with fluorophore labeled concanavalin A (ConA) was assayed by fluorescence microscopy. The fluorescence of those brush polymers was in comparison with glycan arrays composed of monolayers of α-mannosides and α-glucosides ready by combining polymer pen lithography (PPL) with the thiol-ene photochemical response or the copper-catalyzed azide-alkyne cycloaddition. At excessive ConA focus, the fluorescence sign of the brush polymer was practically 20 occasions better than that of the glycan monolayers, and the comb polymer arrays had a detection restrict practically two orders of magnitude higher than their monolayer counterparts.

Due to the potential of this technique to manage exactly the polymer size, the connection between restrict of detection and multivalency could possibly be explored, and it was discovered that the longer polymers (136 nm) are an order of magnitude extra delicate in the direction of ConA binding than the shorter polymers (Eight nm) and that binding affinity decreased systematically with size. These glycan arrays are a brand new software to check the function of multivalency on carbohydrate recognition, and the photopolymerization route in the direction of forming multivalent glycan scaffolds described herein, is a promising path to create multiplexed glycan arrays with nanoscale function dimensions.