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Mixture of Antibody Arrays to Functionally Characterize Darkish Proteins in Human Olfactory Neuroepithelial Cells

The completion and annotation of the human proteome require the provision of knowledge associated to protein perform. Presently, greater than 1800 human genes represent the “darkish proteome,” which embody lacking proteins, uncharacterized human genes validated at protein degree, smORFs, proteins from lncRNAs, or any uncharacterized transcripts. Over the past years, totally different experimental workflows primarily based on multi-omics analyses, bioinformatics, and in vitro and in vivo research have been promoted by the Human Proteome Challenge Consortium to boost the annotation of darkish proteins.

On this chapter, we describe a technique that makes use of recombinant proteins and antibody arrays to ascertain an easy methodology in an effort to quickly characterize potential practical options of darkish proteins related to intracellular signaling dynamics and extracellular immune response in human cell cultures. Additional validating the strategy, this workflow was utilized to probe adjustments within the activation patterns of kinases and transcription components in addition to in cytokine manufacturing modulated by the darkish C1orf128 (PITHD1) protein in human olfactory neuroepithelial cells.

Identification of Antibody Biomarker Utilizing Excessive-Density Nucleic Acid Programmable Protein Array

A novel protein microarray expertise, known as high-density nucleic acid programmable protein array (HD-NAPPA), allows the serological screening of hundreds of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a standard glass microscope slide. By comparability, HD-NAPPA shows proteins in over 10,000 nanowells etched in a silicon slide.

Proteins on HD-NAPPA are expressed within the particular person remoted nanowells, through in vitro transcription and translation (IVTT), with none diffusion throughout incubation. Right here we describe the strategy for antibody biomarker identification utilizing HD-NAPPA, together with 4 major steps: (1) HD-NAPPA array protein expression, (2) main antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) picture scanning and information processing.

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SUFU Antibody

DF7687-200ul 200ul
EUR 350

SUFU antibody

E39-08373 100ug/100ul
EUR 225
Description: Available in various conjugation types.

SUFU antibody

E39-10794 100ug/100ul
EUR 225
Description: Available in various conjugation types.

SUFU Antibody

E309471 200ul
EUR 275
Description: Available in various conjugation types.

SUFU Antibody

35993 100ul
EUR 319

SUFU Antibody

35993-100ul 100ul
EUR 302.4

SUFU antibody

70R-20639 50 ul
EUR 289
Description: Rabbit polyclonal SUFU antibody

SUFU Antibody

ABD7687 100 ug
EUR 525.6

SUFU Antibody

ABD7828 100 ug
EUR 525.6

SUFU Antibody

1-CSB-PA022923GA01HU
  • Ask for price
  • Ask for price
  • 150ul
  • 50ul
Description: A polyclonal antibody against SUFU. Recognizes SUFU from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC

SUFU Antibody

1-CSB-PA891560ESR2HU
  • Ask for price
  • Ask for price
  • 100ul
  • 50ul
Description: A polyclonal antibody against SUFU. Recognizes SUFU from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200

SUFU Antibody

GWB-ML480C 50ug Ask for price

SUFU Antibody

GWB-ML481D 50ug Ask for price

SUFU Antibody

GWB-MN004D 50ug Ask for price

anti- SUFU antibody

FNab08373 100µg
EUR 658.5
Description: Antibody raised against SUFU

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

20-abx322260
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  • Ask for price
  • 100 ul
  • 50 ul

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody

20-abx211712
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  • 100 ul
  • 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

20-abx127058
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  • Ask for price
  • 100 ul
  • 200 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx238373-100ug 100 ug
EUR 610.8

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

20-abx141906
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  • Ask for price
  • 100 ul
  • 200 ul
  • 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx145714-100ug 100 ug
EUR 469.2

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

20-abx005181
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  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx005181-100l 100 µl
EUR 400

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx005181-20l 20 µl
EUR 175

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx005181-50l 50 µl
EUR 275

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx145714-1096tests 10 × 96 tests Ask for price

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx145714-596tests 5 × 96 tests Ask for price

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx145714-96tests 96 tests
EUR 337.5

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx238373-100g 100 µg
EUR 350

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx218817-100tests 100 tests Ask for price

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx218817-200tests 200 tests Ask for price

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx218817-20tests 20 tests
EUR 350

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody

abx211712-100l 100 µl
EUR 350

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody

abx211712-50l 50 µl
EUR 250

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx322260-100g 100 µg
EUR 350

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx322260-50g 50 µg
EUR 237.5

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody

abx433613-100g 100 µg
EUR 387.5

OACA07036-50UL - SUFU Antibody

OACA07036-50UL 50ul
EUR 189

OAAN01995-50UL - SUFU Antibody

OAAN01995-50UL 50ul
EUR 149

OACA07036-100UL - SUFU Antibody

OACA07036-100UL 100ul
EUR 230

OAGA04566-100UL - SUFU Antibody

OAGA04566-100UL 100ul
EUR 369

OAAN01995-100UL - SUFU Antibody

OAAN01995-100UL 100ul
EUR 249

OAAN01995-200UL - SUFU Antibody

OAAN01995-200UL 200ul
EUR 389

OAAL00696-100UG - SUFU Antibody

OAAL00696-100UG 100ug
EUR 349

SUFU (pS342) Antibody

20-abx000504
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  • 100 ul
  • 200 ul
  • 50 ul

SUFU Polyclonal Antibody

E96757 100ul
EUR 225
Description: Available in various conjugation types.

SUFU Polyclonal Antibody

E913429 100ul
EUR 225
Description: Available in various conjugation types.

SUFU Conjugated Antibody

C35993 100ul
EUR 476.4

SUFU Conjugated Antibody

C39155 100ul
EUR 476.4

Suppressor of Fused (SUFU) Antibody

E8RT1600 100ul
EUR 225
Description: Available in various conjugation types.

Polyclonal SUFU Antibody (C-term)

APR04889G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU (C-term). This antibody is tested and proven to work in the following applications:

Polyclonal SUFU antibody - middle region

APR00655G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications:

Polyclonal SUFU antibody - middle region

APR00749G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications:

Monoclonal SUFU Antibody, Clone: 1783CT536.263.29

AMM02579G 0.1ml
EUR 580.8
Description: A Monoclonal antibody against Human SUFU. The antibodies are raised in Mouse and are from clone 1783CT536.263.29. This antibody is applicable in WB, E

Sufu (Phospho-Ser342) Antibody

11552 100ul
EUR 319

Sufu (Phospho-Ser342) Antibody

11552-100ul 100ul
EUR 302.4

Sufu (Phospho-Ser342) Antibody

11552-50ul 50ul
EUR 224.4

Sufu (Phospho-Ser342) Antibody

E011552 100μg/100μl
EUR 225
Description: Available in various conjugation types.

SUFU (Phospho-Ser346) Antibody

SAB260P 100ul
EUR 319

SUFU Mouse Monoclonal Antibody

E10G34348 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

SUFU Rabbit Polyclonal Antibody

E10G00795 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Polyclonal SUFU antibody - N-terminal region

APR00623G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - N-terminal region. This antibody is tested and proven to work in the following applications:

ARP33018_P050-25UL - SUFU Antibody - middle region

ARP33018_P050-25UL 25ul
EUR 99

ARP87204_P050-25UL - SUFU Antibody - middle region

ARP87204_P050-25UL 25ul
EUR 99

ARP87205_P050-25UL - SUFU Antibody - middle region

ARP87205_P050-25UL 25ul
EUR 99

ARP39233_P050-25UL - SUFU Antibody - middle region

ARP39233_P050-25UL 25ul
EUR 99

ARP33018_P050 - SUFU antibody - middle region (ARP33018_P050)

ARP33018_P050 100ul
EUR 389

ARP87204_P050 - SUFU Antibody - middle region (ARP87204_P050)

ARP87204_P050 100ul
EUR 389

ARP87205_P050 - SUFU Antibody - middle region (ARP87205_P050)

ARP87205_P050 100ul
EUR 389

ARP39233_P050 - SUFU antibody - middle region (ARP39233_P050)

ARP39233_P050 100ul
EUR 389

SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody

abx332917-100ul 100 ul
EUR 560.4

SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody

abx332917-100g 100 µg Ask for price

SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody

abx332917-20g 20 µg
EUR 412.5

SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody

abx332917-50g 50 µg Ask for price

Monoclonal SUFU Antibody (monoclonal) (M01), Clone: 1B2

AMM04155G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human SUFU (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B2. This antibody is applicable in E

ARP33019_P050-25UL - SUFU Antibody - N-terminal region

ARP33019_P050-25UL 25ul
EUR 99

SUFU

E8ER1802-68 100ul
EUR 275
Description: Available in various conjugation types.

SUFU

CSB-CL891560HU 10 μg plasmid + 200μl Glycerol Ask for price

SUFU

pro-938 5µg
EUR 60
Description: Recombinant Human Suppressor of Fused Homolog

Sufu (Phospho-Ser342) Polyclonal Conjugated Antibody

C11552 100ul
EUR 476.4

SUFU siRNA

20-abx935622
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  • Ask for price
  • 15 nmol
  • 30 nmol

SUFU siRNA

20-abx935623
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  • Ask for price
  • 15 nmol
  • 30 nmol

ARP33019_P050 - SUFU

ARP33019_P050 100ul
EUR 389

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein

20-abx261500
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  • Ask for price
  • Ask for price
  • 1 mg
  • 20 ug
  • 5 ug

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein

abx261500-100g 100 µg
EUR 3550

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein

abx261500-10g 10 µg
EUR 325

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Protein

abx261500-2g 2 µg
EUR 225

Rabbit Anti-Human SUFU Polyclonal Antibody, Phospho-Ser342

CPB-869RH 100 ul
EUR 670.8

SUFU Mouse mAb

E2618296 100ul
EUR 225
Description: Available in various conjugation types.

SUFU Rabbit pAb

E2513429 100ul
EUR 225
Description: Available in various conjugation types.

SUFU Rabbit pAb

E2506757 100ul
EUR 225
Description: Available in various conjugation types.

SUFU Rabbit pAb

A13429-100ul 100 ul
EUR 369.6

SUFU Rabbit pAb

A13429-200ul 200 ul
EUR 550.8

SUFU Rabbit pAb

A13429-20ul 20 ul
EUR 219.6

SUFU Rabbit pAb

A13429-50ul 50 ul
EUR 267.6

SUFU Rabbit pAb

A19438-100ul 100 ul Ask for price

SUFU Rabbit pAb

A19438-200ul 200 ul Ask for price

SUFU Rabbit pAb

A19438-20ul 20 ul Ask for price

SUFU Rabbit pAb

A19438-50ul 50 ul
EUR 369.6

SUFU Rabbit pAb

A6757-100ul 100 ul
EUR 369.6

SUFU Rabbit pAb

A6757-200ul 200 ul
EUR 550.8

SUFU Rabbit pAb

A6757-20ul 20 ul
EUR 219.6

SUFU Rabbit pAb

A6757-50ul 50 ul
EUR 267.6

SUFU cloning plasmid

CSB-CL891560HU-10ug 10ug
EUR 620.4
Description: A cloning plasmid for the SUFU gene.

SUFU Blocking Peptide

DF7687-BP 1mg
EUR 234

Human SUFU Negative Regulator Of Hedgehog Signaling (SUFU) ELISA Kit

abx383558-96tests 96 tests
EUR 1093.2

SUFU ELISA KIT|Human

EF003347 96 Tests
EUR 826.8

SUFU protein (His tag)

80R-1977 100 ug
EUR 329
Description: Recombinant human SUFU protein (His tag)

Suppressor of Fused (SUFU) pSer342 rabbit polyclonal antibody, Aff - Purified

AP26040PU-N 100 µg Ask for price

Suppressor of Fused (SUFU) pSer342 rabbit polyclonal antibody, Aff - Purified

AP26040PU-S 50 µg Ask for price

Human SUFU shRNA Plasmid

20-abx959898
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  • Ask for price
  • 150 µg
  • 300 µg

Mouse SUFU shRNA Plasmid

20-abx973629
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  • Ask for price
  • 150 µg
  • 300 µg

Sufu (untagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), (10 ug)

RN210578 10 µg Ask for price

SUFU Recombinant Protein (Rat)

RP231659 100 ug Ask for price

SUFU Recombinant Protein (Mouse)

RP176351 100 ug Ask for price

SUFU Recombinant Protein (Mouse)

RP176354 100 ug Ask for price

SUFU Recombinant Protein (Human)

RP030583 100 ug Ask for price

SUFU (C-term) Rabbit pAb

E2619263 100ul
EUR 225
Description: Available in various conjugation types.

Sufu ORF Vector (Rat) (pORF)

ORF077221 1.0 ug DNA
EUR 607.2

SUFU (untagged)-Human suppressor of fused homolog (Drosophila) (SUFU) transcript variant 2

SC328714 10 µg Ask for price

SUFU (untagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 1

SC114403 10 µg Ask for price

SUFU ORF Vector (Human) (pORF)

ORF010195 1.0 ug DNA
EUR 114

Sufu ORF Vector (Mouse) (pORF)

ORF058785 1.0 ug DNA
EUR 607.2

Sufu ORF Vector (Mouse) (pORF)

ORF058786 1.0 ug DNA
EUR 607.2

Human SUFU knockout cell line

ABC-KH14885 1 vial Ask for price
Description: Human SUFU knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Sufu (Myc-DDK-tagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), (10 ug)

RR210578 10 µg Ask for price

Sufu (GFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2

MG207758 10 µg Ask for price

SUFU (GFP-tagged) - Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 1

RG201021 10 µg Ask for price

SUFU (GFP-tagged) - Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 2

RG230076 10 µg Ask for price

Human SUFU knockdown cell line

ABC-KD14885 1 vial Ask for price
Description: Human SUFU knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human SUFU Protein Lysate 20ug

IHUSUFUPLLY20UG each
EUR 211
Description: Human SUFU Protein Lysate 20ug

Sufu (untagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2, (10ug)

MC216599 10 µg Ask for price

Sufu (untagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1, (10ug)

MC216658 10 µg Ask for price

OCOA02270-20UG - SUFU Protein Lysate

OCOA02270-20UG 20ug
EUR 169

Sufu (GFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu) transcript variant 1, (10ug)

MG222908 10 µg Ask for price

SUFU Over-expression Lysate Product

GWB-196C07 0.1 mg Ask for price

SUFU (Myc-DDK-tagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 2

RC230076 10 µg Ask for price

SUFU (Myc-DDK-tagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 1

RC201021 10 µg Ask for price

Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1

MR222908 10 µg Ask for price

Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2

MR207758 10 µg Ask for price

Sufu sgRNA CRISPR Lentivector set (Rat)

K7545701 3 x 1.0 ug
EUR 406.8

Lenti ORF particles, Sufu (GFP-tagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), 200ul, >10^7 TU/mL

RR210578L4V 200 µl Ask for price

Lenti ORF clone of Sufu (mGFP-tagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), (10 ug)

RR210578L4 10 µg Ask for price

SUFU 3'UTR GFP Stable Cell Line

TU074900 1.0 ml
EUR 2799.6

Sufu 3'UTR GFP Stable Cell Line

TU271384 1.0 ml Ask for price

Sufu 3'UTR GFP Stable Cell Line

TU169919 1.0 ml Ask for price

SUFU sgRNA CRISPR Lentivector set (Human)

K2311501 3 x 1.0 ug
EUR 406.8

Sufu sgRNA CRISPR Lentivector set (Mouse)

K4415301 3 x 1.0 ug
EUR 406.8

Lenti ORF particles, Sufu (Myc-DDK-tagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), 200ul, >10^7 TU/mL

RR210578L3V 200 µl Ask for price

SUFU Protein Vector (Rat) (pPM-C-HA)

PV308884 500 ng
EUR 723.6

AAP87204-100UG - SUFU Peptide - middle region

AAP87204-100UG 100ug
EUR 99

AAP87205-100UG - SUFU Peptide - middle region

AAP87205-100UG 100ug
EUR 99

AAP39233-100UG - SUFU Peptide - middle region

AAP39233-100UG 100ug
EUR 99

AAP33018-100UG - SUFU Peptide - middle region

AAP33018-100UG 100ug
EUR 99

Lenti ORF clone of Sufu (Myc-DDK-tagged ORF) - Rat suppressor of fused homolog (Drosophila) (Sufu), (10 ug)

RR210578L3 10 µg Ask for price

SUFU Protein Vector (Rat) (pPB-C-His)

PV308882 500 ng
EUR 723.6

SUFU Protein Vector (Rat) (pPB-N-His)

PV308883 500 ng
EUR 723.6

SUFU Protein Vector (Rat) (pPM-C-His)

PV308885 500 ng
EUR 723.6

SUFU Protein Vector (Human) (pPM-C-HA)

PV040779 500 ng
EUR 394.8

SUFU Protein Vector (Mouse) (pPM-C-HA)

PV235140 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPM-C-HA)

PV235144 500 ng
EUR 723.6

Lenti ORF clone of Sufu (mGFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1

MR222908L4 10 µg Ask for price

Lenti ORF clone of Sufu (mGFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2

MR207758L4 10 µg Ask for price

SUFU Protein Vector (Human) (pPB-C-His)

PV040777 500 ng
EUR 394.8

SUFU Protein Vector (Human) (pPB-N-His)

PV040778 500 ng
EUR 394.8

SUFU Protein Vector (Human) (pPM-C-His)

PV040780 500 ng
EUR 394.8

SUFU Protein Vector (Mouse) (pPB-C-His)

PV235138 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPB-N-His)

PV235139 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPM-C-His)

PV235141 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPB-C-His)

PV235142 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPB-N-His)

PV235143 500 ng
EUR 723.6

SUFU Protein Vector (Mouse) (pPM-C-His)

PV235145 500 ng
EUR 723.6

Sufu 3'UTR Luciferase Stable Cell Line

TU119919 1.0 ml Ask for price

SUFU 3'UTR Luciferase Stable Cell Line

TU024900 1.0 ml
EUR 2799.6

Sufu 3'UTR Luciferase Stable Cell Line

TU221384 1.0 ml Ask for price

Lenti-ORF clone of SUFU (mGFP-tagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 2

RC230076L4 10 µg Ask for price

Sufu sgRNA CRISPR Lentivector (Rat) (Target 1)

K7545702 1.0 ug DNA
EUR 184.8

Sufu sgRNA CRISPR Lentivector (Rat) (Target 2)

K7545703 1.0 ug DNA
EUR 184.8

Sufu sgRNA CRISPR Lentivector (Rat) (Target 3)

K7545704 1.0 ug DNA
EUR 184.8

Lenti-ORF clone of SUFU (Myc-DDK-tagged)-Human suppressor of fused homolog (Drosophila) (SUFU), transcript variant 2

RC230076L3 10 µg Ask for price

Lenti ORF clone of Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1

MR222908L3 10 µg Ask for price

Lenti ORF clone of Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2

MR207758L3 10 µg Ask for price

SUFU sgRNA CRISPR Lentivector (Human) (Target 1)

K2311502 1.0 ug DNA
EUR 184.8

Conduction Band Power-Degree Engineering for Bettering Open-Circuit Voltage in Antimony Selenide Nanorod Array Photo voltaic Cells

Antimony selenide (Sb2 Se3 ) nanorod arrays alongside the [001] orientation are identified to switch photogenerated carriers quickly as a result of strongly anisotropic one-dimensional crystal construction. With superior light-trapping constructions, the Sb2 Se3 nanorod array-based photo voltaic cells have glorious broad spectral response properties, and better short-circuit present density than the traditional planar structured skinny movie photo voltaic cells. Nevertheless, the interface engineering for the Sb2 Se3 nanorod array-based photo voltaic cell is extra essential to extend the efficiency, as a result of it’s difficult to coat a compact buffer layer with good protection to type a uniform heterojunction interface attributable to its massive floor space and length-diameter ratio.

On this work, an intermeshing In2 S3 nanosheet-CdS composite because the buffer layer, compactly coating on the Sb2 Se3 nanorod floor is constructed. The appliance of In2 S3 -CdS composite buffers construct a gradient conduction band power configuration within the Sb2 Se3 /buffer heterojunction interface, which reduces the interface recombination and enhances the switch and assortment of photogenerated electrons. The energy-level regulation minimizes the open-circuit voltage deficit on the interfaces of buffer/Sb2 Se3 and buffer/ZnO layers within the Sb2 Se3 photo voltaic cells. Consequently, the Sb2 Se3 nanorod array photo voltaic cell primarily based on In2 S3 -CdS composite buffers achieves an effectivity of as excessive as 9.19% with a VOC of 461 mV.

Distinguishing between Isomeric Neoxanthin and Violaxanthin Esters in Yellow Flower Petals utilizing Liquid Chromatography-Photodiode Array Atmospheric Stress Chemical Ionization Mass Spectrometry and MS/MS

Rationale: Liquid chromatography-photodiode array atmospheric strain chemical ionization mass spectrometry (LC-PDA-APCI-MS) is used for the evaluation of assorted carotenoid pigments in crops. Amongst them, it’s tough to differentiate between the isomeric violaxanthin/neoxanthin esters.

Strategies: The yellow pigments of tomato petals have been extracted with acetone, and the extracts have been saved at -30°C to settle out the contaminating triacylglycerols bodily. The supernatants have been analyzed utilizing LC-PDA-APCI-MS with a high-resolution orbitrap MS for his or her precise lots. The anticipated carotenoid esters have been calculated with the mixture of carotenoids and fatty acids, they usually have been matched with the experimental precise lots. The fatty acid constructions within the carotenoid esters have been additionally recognized utilizing collision-induced dissociation (CID) MS/MS. The isomeric violaxanthin/neoxanthin esters have been distinguished utilizing CID MS/MS from their in-source dehydrated product ions as pseudoprecursor ions.

Outcomes: The in-source dehydrated ions [M-H2 O+H]+ of neoxanthin diesters predominated over their protonated molecules [M+H]+ within the LC-MS. Against this, the protonated molecules of violaxanthin diesters predominated. The 92 u loss product ions [M-H2 O-C7 H8 +H]+ have been noticed from the dehydrated violaxanthin diesters, however they weren’t generated from the dehydrated neoxanthin diesters within the CID MS/MS of their dehydrated pseudoprecursor ion [M-H2 O+H]+ .

Conclusions: The allene allyl carbocation in neoxanthin diesters was generated from dehydration after preferential protonation on the hydroxy group. The epoxide group of violaxanthin diesters opens simply after protonation; nonetheless, the dehydration didn’t proceed at this stage. The 92 u lack of C7 H8 was defined by the intramolecular [2+2] cycloaddition, which proceeded preferentially in dehydrated violaxanthin diesters as a result of the carbocations within the dehydrated species have been conjugated to the polyene and people double bonds have been depolarized within the CID MS/MS. Due to this fact, the isomeric neoxanthin/violaxanthin diesters have been distinguished utilizing LC-PDA-APCI-MS and MS/MS. This technique was a sensible and helpful technique of profiling the carotenoid esters of the yellow petals.

Beam pen lithography as a brand new software for spatially managed photochemistry, and its utilization within the synthesis of multivalent glycan arrays

Herein, we describe how cantilever-free scanning probes can be utilized to deposit precursor materials and subsequently irradiate the precursor to provoke polymerization, leading to a 3D lithographic technique whereby the place, peak and diameter of every function may be tuned independently. Particularly, acrylate and methacrylate monomers have been patterned onto thiol terminated glass and subsequently uncovered to UV mild produced brush polymers by a photoinduced radical acrylate polymerization response. Right here, we report the primary examples of glycan arrays, comprised of methacrylate brush polymers which are side-chain functionalized with α-glucose, by this new lithographic method.

Their binding with fluorophore labeled concanavalin A (ConA) was assayed by fluorescence microscopy. The fluorescence of those brush polymers was in comparison with glycan arrays composed of monolayers of α-mannosides and α-glucosides ready by combining polymer pen lithography (PPL) with the thiol-ene photochemical response or the copper-catalyzed azide-alkyne cycloaddition. At excessive ConA focus, the fluorescence sign of the brush polymer was practically 20 occasions better than that of the glycan monolayers, and the comb polymer arrays had a detection restrict practically two orders of magnitude higher than their monolayer counterparts.

Due to the potential of this technique to manage exactly the polymer size, the connection between restrict of detection and multivalency could possibly be explored, and it was discovered that the longer polymers (136 nm) are an order of magnitude extra delicate in the direction of ConA binding than the shorter polymers (Eight nm) and that binding affinity decreased systematically with size. These glycan arrays are a brand new software to check the function of multivalency on carbohydrate recognition, and the photopolymerization route in the direction of forming multivalent glycan scaffolds described herein, is a promising path to create multiplexed glycan arrays with nanoscale function dimensions.

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