Mixture of Antibody Arrays to Functionally Characterize Darkish Proteins in Human Olfactory Neuroepithelial Cells

The completion and annotation of the human proteome require the provision of knowledge associated to protein perform. Presently, greater than 1800 human genes represent the “darkish proteome,” which embody lacking proteins, uncharacterized human genes validated at protein degree, smORFs, proteins from lncRNAs, or any uncharacterized transcripts. Over the past years, totally different experimental workflows primarily based on multi-omics analyses, bioinformatics, and in vitro and in vivo research have been promoted by the Human Proteome Challenge Consortium to boost the annotation of darkish proteins.

On this chapter, we describe a technique that makes use of recombinant proteins and antibody arrays to ascertain an easy methodology in an effort to quickly characterize potential practical options of darkish proteins related to intracellular signaling dynamics and extracellular immune response in human cell cultures. Additional validating the strategy, this workflow was utilized to probe adjustments within the activation patterns of kinases and transcription components in addition to in cytokine manufacturing modulated by the darkish C1orf128 (PITHD1) protein in human olfactory neuroepithelial cells.

Identification of Antibody Biomarker Utilizing Excessive-Density Nucleic Acid Programmable Protein Array

A novel protein microarray expertise, known as high-density nucleic acid programmable protein array (HD-NAPPA), allows the serological screening of hundreds of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a standard glass microscope slide. By comparability, HD-NAPPA shows proteins in over 10,000 nanowells etched in a silicon slide.

Proteins on HD-NAPPA are expressed within the particular person remoted nanowells, through in vitro transcription and translation (IVTT), with none diffusion throughout incubation. Right here we describe the strategy for antibody biomarker identification utilizing HD-NAPPA, together with 4 major steps: (1) HD-NAPPA array protein expression, (2) main antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) picture scanning and information processing.

Sufu Antibody
CSB-PA022923XA01MO-02mg	Cusabio	0.2mg	Ask for price
Description: Recombinant Mus musculus (Mouse) Sufu protein

Sufu Antibody
CSB-PA022923XA01MO-10mg	Cusabio	10mg	Ask for price
Description: Recombinant Mus musculus (Mouse) Sufu protein

SUFU Antibody
DF7687	Affbiotech	200ul	EUR 420

SUFU Antibody
DF7687-100ul	Affinity Biosciences	100ul	EUR 168
Description: WB,IHC,ELISA(peptide)

SUFU Antibody
DF7687-200ul	Affinity Biosciences	200ul	EUR 210
Description: WB,IHC,ELISA(peptide)

SUFU Antibody
E035993	EnoGene	100μg/100μl	EUR 255
Description: Available in various conjugation types.

SUFU Antibody
1-CSB-PA891560ESR2HU	Cusabio	Ask for price Ask for price	100ul 50ul
Description: A polyclonal antibody against SUFU. Recognizes SUFU from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200

sufu Antibody
E93273	EnoGene	100ul	EUR 255
Description: Available in various conjugation types.

SUFU Antibody
E45M02579G-1	EnoGene	100 ul	EUR 395

SUFU Antibody
E45M02579G-4	EnoGene	50 ul	EUR 295

SUFU Antibody
E309471	EnoGene	200ul	EUR 275
Description: Available in various conjugation types.

SUFU Antibody
E19-7687-1	EnoGene	50ug/50ul	EUR 145
Description: Available in various conjugation types.

SUFU Antibody
E19-7687-2	EnoGene	100ug/100ul	EUR 225
Description: Available in various conjugation types.

SUFU antibody
E39-08373	EnoGene	100ug/100ul	EUR 225
Description: Available in various conjugation types.

SUFU antibody
E39-10794	EnoGene	100ug/100ul	EUR 225
Description: Available in various conjugation types.

SUFU Antibody
GWB-ML480C	GenWay Biotech	50ug	Ask for price

SUFU Antibody
GWB-ML481D	GenWay Biotech	50ug	Ask for price

SUFU Antibody
GWB-MN004D	GenWay Biotech	50ug	Ask for price

Sufu antibody
MBS5309640-005mg	MyBiosource	0.05mg	EUR 485

Sufu antibody
MBS5309640-5x005mg	MyBiosource	5x0.05mg	EUR 2045

SUFU Antibody
MBS7124473-005mL	MyBiosource	0.05mL	EUR 190

SUFU Antibody
MBS7124473-01mL	MyBiosource	0.1mL	EUR 270

SUFU Antibody
MBS7124473-5x01mL	MyBiosource	5x0.1mL	EUR 1205

SUFU Antibody
MBS8517065-005mg	MyBiosource	0.05mg	EUR 235

SUFU Antibody
MBS8517065-01mg	MyBiosource	0.1mg	EUR 305

SUFU Antibody
MBS8517065-01mLAF405M	MyBiosource	0.1mL(AF405M)	EUR 465

SUFU Antibody
MBS8517065-01mLAF546	MyBiosource	0.1mL(AF546)	EUR 465

SUFU Antibody
MBS8517065-01mLAF750	MyBiosource	0.1mL(AF750)	EUR 465

sufu Antibody
MBS8505037-01mL	MyBiosource	0.1mL	EUR 325

sufu Antibody
MBS8505037-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

sufu Antibody
MBS8505037-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

sufu Antibody
MBS8505037-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

sufu Antibody
MBS8505037-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

SUFU Antibody
MBS9405216-01mL	MyBiosource	0.1mL	EUR 305

SUFU Antibody
MBS9405216-5x01mL	MyBiosource	5x0.1mL	EUR 1230

SUFU Antibody
MBS9230955-005mL	MyBiosource	0.05mL	EUR 210

SUFU Antibody
MBS9230955-02mL	MyBiosource	0.2mL	EUR 430

SUFU Antibody
MBS9230955-5x02mL	MyBiosource	5x0.2mL	EUR 1910

SUFU Antibody
MBS9608269-01mL	MyBiosource	0.1mL	EUR 260

SUFU Antibody
MBS9608269-02mL	MyBiosource	0.2mL	EUR 305

SUFU Antibody
MBS9608269-5x02mL	MyBiosource	5x0.2mL	EUR 1220

SUFU antibody
MBS9414663-005mL	MyBiosource	0.05mL	EUR 300

SUFU antibody
MBS9414663-01mL	MyBiosource	0.1mL	EUR 390

SUFU antibody
MBS9414663-5x01mL	MyBiosource	5x0.1mL	EUR 1610

anti- SUFU antibody
FNab08373	FN Test	100µg	EUR 658.5
Description: Antibody raised against SUFU

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx005181-100l	Abbexa	100 µl	EUR 400

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
20-abx005181	Abbexa	Ask for price Ask for price Ask for price Ask for price	20 ul 50 ul 100 ul 200 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx005181-20l	Abbexa	20 µl	EUR 175

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx005181-50l	Abbexa	50 µl	EUR 275

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
20-abx127058	Abbexa	Ask for price Ask for price	100 ul 200 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
20-abx141906	Abbexa	Ask for price Ask for price Ask for price	50 ul 100 ul 200 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx145714-100ug	Abbexa	100 ug	EUR 469.2

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx145714-1096tests	Abbexa	10 × 96 tests	Ask for price

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx145714-596tests	Abbexa	5 × 96 tests	Ask for price

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx145714-96tests	Abbexa	96 tests	EUR 337.5

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx433613-100g	Abbexa	100 µg	EUR 387.5

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx322260-100g	Abbexa	100 µg	EUR 350

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
20-abx322260	Abbexa	Ask for price Ask for price	50 ul 100 ul

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx322260-50g	Abbexa	50 µg	EUR 237.5

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx218817-100tests	Abbexa	100 tests	Ask for price

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx218817-200tests	Abbexa	200 tests	Ask for price

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx218817-20tests	Abbexa	20 tests	EUR 350

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody
abx211712-100l	Abbexa	100 µl	EUR 350

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody
20-abx211712	Abbexa	Ask for price Ask for price	50 ul 100 ul

SUFU Negative Regulator Of Hedgehog Signaling (SUFU) Antibody
abx211712-50l	Abbexa	50 µl	EUR 250

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx238373-100g	Abbexa	100 µg	EUR 350

SUFU Negative Regulator of Hedgehog Signaling (SUFU) Antibody
abx238373-100ug	Abbexa	100 ug	EUR 610.8

SUFU Antibody (C-term)
E45R04889G-1	EnoGene	100 ul	EUR 395

SUFU Antibody (C-term)
E45R04889G-4	EnoGene	50 ul	EUR 295

SUFU Antibody (C-term)
MBS9215763-005mL	MyBiosource	0.05mL	EUR 210

SUFU Antibody (C-term)
MBS9215763-02mL	MyBiosource	0.2mL	EUR 430

SUFU Antibody (C-term)
MBS9215763-5x02mL	MyBiosource	5x0.2mL	EUR 1910

SUFU Antibody - middle region
MBS3223163-01mL	MyBiosource	0.1mL	EUR 455

SUFU Antibody - middle region
MBS3223163-5x01mL	MyBiosource	5x0.1mL	EUR 1995

SUFU Antibody - middle region
MBS3223164-01mL	MyBiosource	0.1mL	EUR 455

SUFU Antibody - middle region
MBS3223164-5x01mL	MyBiosource	5x0.1mL	EUR 1995

SUFU antibody - middle region
MBS3201285-01mL	MyBiosource	0.1mL	EUR 455

SUFU antibody - middle region
MBS3201285-5x01mL	MyBiosource	5x0.1mL	EUR 1995

SUFU antibody - middle region
MBS3204508-01mL	MyBiosource	0.1mL	EUR 455

SUFU antibody - middle region
MBS3204508-5x01mL	MyBiosource	5x0.1mL	EUR 1995

OAAN01995-50UL - SUFU Antibody
OAAN01995-50UL	Aviva Systems Biology	50ul	EUR 149

OACA07036-50UL - SUFU Antibody
OACA07036-50UL	Aviva Systems Biology	50ul	EUR 189

OAAL00696-100UG - SUFU Antibody
OAAL00696-100UG	Aviva Systems Biology	100ug	EUR 349

OAAN01995-100UL - SUFU Antibody
OAAN01995-100UL	Aviva Systems Biology	100ul	EUR 249

OAAN01995-200UL - SUFU Antibody
OAAN01995-200UL	Aviva Systems Biology	200ul	EUR 389

OAGA04566-100UL - SUFU Antibody
OAGA04566-100UL	Aviva Systems Biology	100ul	EUR 369

OACA07036-100UL - SUFU Antibody
OACA07036-100UL	Aviva Systems Biology	100ul	EUR 230

SUFU (pS342) Antibody
20-abx000504	Abbexa	Ask for price Ask for price Ask for price	50 ul 100 ul 200 ul

SUFU Conjugated Antibody
C35993	SAB	100ul	EUR 476.4

SUFU polyclonal antibody
BS91299	Bioworld Biotech	50ul	EUR 398
Description: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

SUFU polyclonal antibody
BS8416	Bioworld Biotech	50ul	EUR 358
Description: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

SUFU Conjugated Antibody
C39155	SAB	100ul	EUR 476.4

SUFU Polyclonal Antibody
MBS129948-002mL	MyBiosource	0.02mL	EUR 190

SUFU Polyclonal Antibody
MBS129948-005mL	MyBiosource	0.05mL	EUR 235

SUFU Polyclonal Antibody
MBS129948-01mL	MyBiosource	0.1mL	EUR 320

SUFU Polyclonal Antibody
MBS129948-02mL	MyBiosource	0.2mL	EUR 500

SUFU Polyclonal Antibody
MBS129948-5x02mL	MyBiosource	5x0.2mL	EUR 2065

SUFU Polyclonal Antibody
E913429	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

SUFU Polyclonal Antibody
E96757	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

SUFU monoclonal antibody
MB67251	Bioworld Biotech	50ul	EUR 275
Description: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

SUFU Polyclonal Antibody
MBS2555007-006mL	MyBiosource	0.06mL	EUR 190

SUFU Polyclonal Antibody
MBS2555007-012mL	MyBiosource	0.12mL	EUR 265

SUFU Polyclonal Antibody
MBS2555007-02mL	MyBiosource	0.2mL	EUR 415

SUFU Polyclonal Antibody
MBS2555007-5x02mL	MyBiosource	5x0.2mL	EUR 1835

SUFU Polyclonal Antibody
MBS9133470-002mL	MyBiosource	0.02mL	EUR 200

SUFU Polyclonal Antibody
MBS9133470-005mL	MyBiosource	0.05mL	EUR 255

SUFU Polyclonal Antibody
MBS9133470-01mL	MyBiosource	0.1mL	EUR 345

SUFU Polyclonal Antibody
MBS9133470-02mL	MyBiosource	0.2mL	EUR 545

SUFU Polyclonal Antibody
MBS9133470-5x02mL	MyBiosource	5x0.2mL	EUR 2265

SUFU Polyclonal Antibody
MBS8573078-01mL	MyBiosource	0.1mL	EUR 305

SUFU Polyclonal Antibody
MBS8573078-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

SUFU Polyclonal Antibody
MBS8573078-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

SUFU Polyclonal Antibody
MBS8573078-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

SUFU Polyclonal Antibody
MBS8573078-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

SUFU Conjugated Antibody
MBS9449197-01mLAF350	MyBiosource	0.1mL(AF350)	EUR 480

SUFU Conjugated Antibody
MBS9449197-01mLAF405	MyBiosource	0.1mL(AF405)	EUR 480

SUFU Conjugated Antibody
MBS9449197-01mLAF488	MyBiosource	0.1mL(AF488)	EUR 480

SUFU Conjugated Antibody
MBS9449197-01mLAF555	MyBiosource	0.1mL(AF555)	EUR 480

SUFU Conjugated Antibody
MBS9449197-01mLBiotin	MyBiosource	0.1mL(Biotin)	EUR 480

SUFU Conjugated Antibody
MBS9446456-01mLAF350	MyBiosource	0.1mL(AF350)	EUR 480

SUFU Conjugated Antibody
MBS9446456-01mLAF405	MyBiosource	0.1mL(AF405)	EUR 480

SUFU Conjugated Antibody
MBS9446456-01mLAF488	MyBiosource	0.1mL(AF488)	EUR 480

SUFU Conjugated Antibody
MBS9446456-01mLAF555	MyBiosource	0.1mL(AF555)	EUR 480

SUFU Conjugated Antibody
MBS9446456-01mLBiotin	MyBiosource	0.1mL(Biotin)	EUR 480

SUFU Polyclonal Antibody
MBS8568160-01mL	MyBiosource	0.1mL	EUR 305

SUFU Polyclonal Antibody
MBS8568160-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

SUFU Polyclonal Antibody
MBS8568160-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

SUFU Polyclonal Antibody
MBS8568160-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

SUFU Polyclonal Antibody
MBS8568160-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

SUFU Polyclonal Antibody
RD81405A-120uL	Reddot Biotech	120μL	EUR 360
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

SUFU Polyclonal Antibody
RD81405A-200uL	Reddot Biotech	200μL	EUR 630
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

SUFU Polyclonal Antibody
RD81405A-60uL	Reddot Biotech	60μL	EUR 204
Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Human, Rat, Mouse SUFU antibody
P0696	Omnimabs	100ul	EUR 150

Polyclonal SUFU Antibody (C-term)
APR04889G	Leading Biology	0.1ml	EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU (C-term). This antibody is tested and proven to work in the following applications:

Suppressor of Fused (SUFU) Antibody
E8RT1600	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

SUFU antibody - N-terminal region
MBS3201286-01mL	MyBiosource	0.1mL	EUR 455

SUFU antibody - N-terminal region
MBS3201286-5x01mL	MyBiosource	5x0.1mL	EUR 1995

Polyclonal SUFU antibody - middle region
APR00655G	Leading Biology	0.05mg	EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications:

Polyclonal SUFU antibody - middle region
APR00749G	Leading Biology	0.05mg	EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - middle region. This antibody is tested and proven to work in the following applications:

Monoclonal SUFU Antibody, Clone: 1783CT536.263.29
AMM02579G	Leading Biology	0.1ml	EUR 580.8
Description: A Monoclonal antibody against Human SUFU. The antibodies are raised in Mouse and are from clone 1783CT536.263.29. This antibody is applicable in WB, E

Sufu (Phospho-Ser342) Antibody
11552	SAB	100ul	EUR 319

Sufu (Phospho-Ser342) Antibody
11552-100ul	SAB	100ul	EUR 302.4

Sufu (Phospho-Ser342) Antibody
11552-50ul	SAB	50ul	EUR 224.4

Sufu(Phospho-Ser342) Antibody
E011552	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

SUFU Mouse Monoclonal Antibody
E10G34348	EnoGene	100 μl	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Sufu (Phospho-Ser342) Antibody
MBS9403378-005mL	MyBiosource	0.05mL	EUR 245

Sufu (Phospho-Ser342) Antibody
MBS9403378-01mL	MyBiosource	0.1mL	EUR 305

Sufu (Phospho-Ser342) Antibody
MBS9403378-5x01mL	MyBiosource	5x0.1mL	EUR 1230

SUFU (Phospho-Ser346) Antibody
MBS9458452-005mL	MyBiosource	0.05mL	EUR 245

SUFU (Phospho-Ser346) Antibody
MBS9458452-01mL	MyBiosource	0.1mL	EUR 305

SUFU (Phospho-Ser346) Antibody
MBS9458452-5x01mL	MyBiosource	5x0.1mL	EUR 1230

SUFU (Phospho-Ser346) Antibody
SAB260P	SAB	100ul	EUR 319

SUFU Rabbit Polyclonal Antibody
E10G00795	EnoGene	100 μl	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Polyclonal SUFU antibody - N-terminal region
APR00623G	Leading Biology	0.05mg	EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SUFU - N-terminal region. This antibody is tested and proven to work in the following applications:

ARP39233_P050-25UL - SUFU Antibody - middle region
ARP39233_P050-25UL	Aviva Systems Biology	25ul	EUR 99

ARP33018_P050-25UL - SUFU Antibody - middle region
ARP33018_P050-25UL	Aviva Systems Biology	25ul	EUR 99

ARP87204_P050-25UL - SUFU Antibody - middle region
ARP87204_P050-25UL	Aviva Systems Biology	25ul	EUR 99

ARP87205_P050-25UL - SUFU Antibody - middle region
ARP87205_P050-25UL	Aviva Systems Biology	25ul	EUR 99

ARP39233_P050 - SUFU antibody - middle region (ARP39233_P050)
ARP39233_P050	Aviva Systems Biology	100ul	EUR 389

ARP33018_P050 - SUFU antibody - middle region (ARP33018_P050)
ARP33018_P050	Aviva Systems Biology	100ul	EUR 389

ARP87204_P050 - SUFU Antibody - middle region (ARP87204_P050)
ARP87204_P050	Aviva Systems Biology	100ul	EUR 389

ARP87205_P050 - SUFU Antibody - middle region (ARP87205_P050)
ARP87205_P050	Aviva Systems Biology	100ul	EUR 389

SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody
abx332917-100g	Abbexa	100 µg	Ask for price

SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody
abx332917-100ul	Abbexa	100 ul	EUR 560.4

SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody
abx332917-20g	Abbexa	20 µg	EUR 412.5

SUFU Negative Regulator Of Hedgehog Signaling Phospho-Ser342 (SUFU pS342) Antibody
abx332917-50g	Abbexa	50 µg	Ask for price

Monoclonal SUFU Antibody (monoclonal) (M01), Clone: 1B2
AMM04155G	Leading Biology	0.1mg	EUR 580.8
Description: A Monoclonal antibody against Human SUFU (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B2. This antibody is applicable in E

SUFU (12X11) Rabbit Monoclonal Antibody
E28M3837	EnoGene	100ul	EUR 295

ARP33019_P050-25UL - SUFU Antibody - N-terminal region
ARP33019_P050-25UL	Aviva Systems Biology	25ul	EUR 99

SUFU
CSB-CL891560HU	Cusabio	10 μg plasmid + 200μl Glycerol	Ask for price

SUFU
E8ER1802-68	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

SUFU
MBS8565358-01mL	MyBiosource	0.1mL	EUR 345

SUFU
MBS8565358-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

SUFU
MBS8565358-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

SUFU
MBS8565358-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

SUFU
MBS8565358-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

SUFU
pro-938	ProSpec Tany	5µg	EUR 60
Description: Recombinant Human Suppressor of Fused Homolog

SUFU
MBS8561830-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

SUFU
MBS8561830-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

SUFU
MBS8561830-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

SUFU
MBS8561830-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

SUFU
MBS8561830-02mL	MyBiosource	0.2mL	EUR 345

SUFU Rabbit anti-Human Polyclonal Antibody
MBS2402439-005mL	MyBiosource	0.05mL	EUR 615

Conduction Band Power-Degree Engineering for Bettering Open-Circuit Voltage in Antimony Selenide Nanorod Array Photo voltaic Cells

Antimony selenide (Sb₂ Se₃ ) nanorod arrays alongside the [001] orientation are identified to switch photogenerated carriers quickly as a result of strongly anisotropic one-dimensional crystal construction. With superior light-trapping constructions, the Sb₂ Se₃ nanorod array-based photo voltaic cells have glorious broad spectral response properties, and better short-circuit present density than the traditional planar structured skinny movie photo voltaic cells. Nevertheless, the interface engineering for the Sb₂ Se₃ nanorod array-based photo voltaic cell is extra essential to extend the efficiency, as a result of it’s difficult to coat a compact buffer layer with good protection to type a uniform heterojunction interface attributable to its massive floor space and length-diameter ratio.

On this work, an intermeshing In₂ S₃ nanosheet-CdS composite because the buffer layer, compactly coating on the Sb₂ Se₃ nanorod floor is constructed. The appliance of In₂ S₃ -CdS composite buffers construct a gradient conduction band power configuration within the Sb₂ Se₃ /buffer heterojunction interface, which reduces the interface recombination and enhances the switch and assortment of photogenerated electrons. The energy-level regulation minimizes the open-circuit voltage deficit on the interfaces of buffer/Sb₂ Se₃ and buffer/ZnO layers within the Sb₂ Se₃ photo voltaic cells. Consequently, the Sb₂ Se₃ nanorod array photo voltaic cell primarily based on In₂ S₃ -CdS composite buffers achieves an effectivity of as excessive as 9.19% with a V_OC of 461 mV.

Distinguishing between Isomeric Neoxanthin and Violaxanthin Esters in Yellow Flower Petals utilizing Liquid Chromatography-Photodiode Array Atmospheric Stress Chemical Ionization Mass Spectrometry and MS/MS

Rationale: Liquid chromatography-photodiode array atmospheric strain chemical ionization mass spectrometry (LC-PDA-APCI-MS) is used for the evaluation of assorted carotenoid pigments in crops. Amongst them, it’s tough to differentiate between the isomeric violaxanthin/neoxanthin esters.

Strategies: The yellow pigments of tomato petals have been extracted with acetone, and the extracts have been saved at -30°C to settle out the contaminating triacylglycerols bodily. The supernatants have been analyzed utilizing LC-PDA-APCI-MS with a high-resolution orbitrap MS for his or her precise lots. The anticipated carotenoid esters have been calculated with the mixture of carotenoids and fatty acids, they usually have been matched with the experimental precise lots. The fatty acid constructions within the carotenoid esters have been additionally recognized utilizing collision-induced dissociation (CID) MS/MS. The isomeric violaxanthin/neoxanthin esters have been distinguished utilizing CID MS/MS from their in-source dehydrated product ions as pseudoprecursor ions.

Outcomes: The in-source dehydrated ions [M-H₂ O+H]⁺ of neoxanthin diesters predominated over their protonated molecules [M+H]⁺ within the LC-MS. Against this, the protonated molecules of violaxanthin diesters predominated. The 92 u loss product ions [M-H₂ O-C₇ H₈ +H]⁺ have been noticed from the dehydrated violaxanthin diesters, however they weren’t generated from the dehydrated neoxanthin diesters within the CID MS/MS of their dehydrated pseudoprecursor ion [M-H₂ O+H]⁺ .

Conclusions: The allene allyl carbocation in neoxanthin diesters was generated from dehydration after preferential protonation on the hydroxy group. The epoxide group of violaxanthin diesters opens simply after protonation; nonetheless, the dehydration didn’t proceed at this stage. The 92 u lack of C₇ H₈ was defined by the intramolecular [2+2] cycloaddition, which proceeded preferentially in dehydrated violaxanthin diesters as a result of the carbocations within the dehydrated species have been conjugated to the polyene and people double bonds have been depolarized within the CID MS/MS. Due to this fact, the isomeric neoxanthin/violaxanthin diesters have been distinguished utilizing LC-PDA-APCI-MS and MS/MS. This technique was a sensible and helpful technique of profiling the carotenoid esters of the yellow petals.

Beam pen lithography as a brand new software for spatially managed photochemistry, and its utilization within the synthesis of multivalent glycan arrays

Herein, we describe how cantilever-free scanning probes can be utilized to deposit precursor materials and subsequently irradiate the precursor to provoke polymerization, leading to a 3D lithographic technique whereby the place, peak and diameter of every function may be tuned independently. Particularly, acrylate and methacrylate monomers have been patterned onto thiol terminated glass and subsequently uncovered to UV mild produced brush polymers by a photoinduced radical acrylate polymerization response. Right here, we report the primary examples of glycan arrays, comprised of methacrylate brush polymers which are side-chain functionalized with α-glucose, by this new lithographic method.

Their binding with fluorophore labeled concanavalin A (ConA) was assayed by fluorescence microscopy. The fluorescence of those brush polymers was in comparison with glycan arrays composed of monolayers of α-mannosides and α-glucosides ready by combining polymer pen lithography (PPL) with the thiol-ene photochemical response or the copper-catalyzed azide-alkyne cycloaddition. At excessive ConA focus, the fluorescence sign of the brush polymer was practically 20 occasions better than that of the glycan monolayers, and the comb polymer arrays had a detection restrict practically two orders of magnitude higher than their monolayer counterparts.

Due to the potential of this technique to manage exactly the polymer size, the connection between restrict of detection and multivalency could possibly be explored, and it was discovered that the longer polymers (136 nm) are an order of magnitude extra delicate in the direction of ConA binding than the shorter polymers (Eight nm) and that binding affinity decreased systematically with size. These glycan arrays are a brand new software to check the function of multivalency on carbohydrate recognition, and the photopolymerization route in the direction of forming multivalent glycan scaffolds described herein, is a promising path to create multiplexed glycan arrays with nanoscale function dimensions.